Key features and details
- Rabbit polyclonal to CD28
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CD28 antibody
See all CD28 primary antibodies
DescriptionRabbit polyclonal to CD28
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Cow, Cat
- WB: Human spleen tissue lysate. IF: Human spleen tissue. IHC-P: Human spleen tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa.|
|IHC-P||Use a concentration of 2 µg/ml.|
|ICC/IF||Use a concentration of 20 µg/ml.|
FunctionInvolved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Tissue specificityExpressed in T-cells and plasma cells, but not in less mature B-cells.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
Human spleen tissue stained for CD28 with ab254580 at 2 µg/ml in immunohistochemical analysis.
Human spleen tissue labeling CD28 using ab254580 at 20 µg/ml in IF.
All lanes : Anti-CD28 antibody (ab254580) at 1 µg/ml
Lane 1 : Human spleen tissue lysate
Lane 2 : Human spleen tissue lysate with blocking peptide
Predicted band size: 25 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254580 has not yet been referenced specifically in any publications.