Key features and details
- Mouse monoclonal [CD28.2] to CD28 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD28 antibody [CD28.2] - Low endotoxin, Azide free
See all CD28 primary antibodies
DescriptionMouse monoclonal [CD28.2] to CD28 - Low endotoxin, Azide free
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Non human primates
Human CD28 transfected murine T cell hybridoma.
- Flow Cyt: CD28 positive lymphocytes.
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One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.20
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride
Concentration information loading...
Purification notesThis purified format is guaranteed to be >90% pure as determined by SDS-PAGE analysis. Endotoxin level is less than or equal to 0.01 EU/ug, as determined by the LaL assay.
- PE Anti-CD28 antibody [CD28.2], prediluted (ab134418)
- FITC Anti-CD28 antibody [CD28.2] (ab134472)
- APC Anti-CD28 antibody [CD28.2], prediluted (ab155372)
- PE/Cy7® Anti-CD28 antibody [CD28.2], prediluted (ab155387)
- PerCP/Cy5.5® Anti-CD28 antibody [CD28.2], prediluted (ab157318)
- FITC Anti-CD28 antibody [CD28.2] (ab234227)
- PE/Cy7® Anti-CD28 antibody [CD28.2] (ab234276)
- Biotin Anti-CD28 antibody [CD28.2] (ab267385)
- APC/Cy7® Anti-CD28 antibody [CD28.2] (ab270676)
- PerCP Anti-CD28 antibody [CD28.2] (ab88034)
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 12 µg/ml.
Use a concentration of 1 - 12 µg/ml.
FunctionInvolved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Tissue specificityExpressed in T-cells and plasma cells, but not in less mature B-cells.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
Flow cytometry surface staining pattern of human peripheral blood stained using ab85986 GAM APC.
Separation of human CD28 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab85986 GAM APC.
ab85986 has been referenced in 9 publications.
- Urso K et al. NFATc3 regulates the transcription of genes involved in T-cell activation and angiogenesis. Blood 118:795-803 (2011). PubMed: 21642596
- Chen ML et al. Antipsychotic drugs suppress the AKT/NF-?B pathway and regulate the differentiation of T-cell subsets. Immunol Lett 140:81-91 (2011). Functional Studies ; Human . PubMed: 21763349
- Scharschmidt E et al. Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol 24:3860-73 (2004). PubMed: 15082780
- Jeong SH et al. Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates. J Virol 78:6995-7003 (2004). PubMed: 15194776
- Marti F et al. Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6. J Immunol 166:197-206 (2001). PubMed: 11123293
- Galea-Lauri J et al. Expression of a variant of CD28 on a subpopulation of human NK cells: implications for B7-mediated stimulation of NK cells. J Immunol 163:62-70 (1999). PubMed: 10384100
- Tazi A et al. Evidence that Langerhans cells in adult pulmonary Langerhans cell histiocytosis are mature dendritic cells: importance of the cytokine microenvironment. J Immunol 163:3511-5 (1999). PubMed: 10477625
- Nunès J et al. CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events. Int Immunol 5:311-5 (1993). PubMed: 8385476
- Nunes J et al. Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity. Biochem J 293 ( Pt 3):835-42 (1993). PubMed: 8394695