Key features and details
- Mouse monoclonal [CD28.2] to CD28 (PE/Cy7®)
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
- Isotype: IgG1
Product nameAnti-CD28 antibody [CD28.2] (PE/Cy7®)
See all CD28 primary antibodies
DescriptionMouse monoclonal [CD28.2] to CD28 (PE/Cy7®)
ConjugationPE/Cy7®. Ex: 496nm, Em: 774nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Non human primates
Tissue, cells or virus corresponding to CD28. (DC188.8.131.52 murine T cell hybridoma transfected with human CD28 cDNA)
- Flow Cytometry: Human peripheral blood.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.0975% Sodium azide
Concentration information loading...
Purification notesab234276 is conjugated with tandem dye PE-Cy™7 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
- Anti-CD28 antibody [CD28.2], prediluted (PE) (ab134418)
- Anti-CD28 antibody [CD28.2] (FITC) (ab134472)
- Anti-CD28 antibody [CD28.2], prediluted (APC) (ab155372)
- Anti-CD28 antibody [CD28.2], prediluted (PE/Cy7®) (ab155387)
- Anti-CD28 antibody [CD28.2], prediluted (PerCP/Cy5.5®) (ab157318)
- Anti-CD28 antibody [CD28.2] (FITC) (ab234227)
- Anti-CD28 antibody [CD28.2] (Biotin) (ab267385)
- Anti-CD28 antibody [CD28.2] (APC/Cy7®) (ab270676)
- Anti-CD28 antibody [CD28.2] - Low endotoxin, Azide free (ab85986)
- Anti-CD28 antibody [CD28.2] (PerCP) (ab88034)
Our Abpromise guarantee covers the use of ab234276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 4µl for 106 cells.|
FunctionInvolved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Tissue specificityExpressed in T-cells and plasma cells, but not in less mature B-cells.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234276 has not yet been referenced specifically in any publications.