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    cd28-antibody-cd282-prediluted-pecy7-ab155387.pdf

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Immunology Adaptive Immunity B Cells CD
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Anti-CD28 antibody [CD28.2], prediluted (PE/Cy7 ®) (ab155387)

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  • Flow Cytometry - Anti-CD28 antibody [CD28.2], prediluted (PE/Cy7 ®) (ab155387)

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Primary

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-CD28 antibody [CD28.2], prediluted (PE/Cy7 ®)
    See all CD28 primary antibodies
  • Description

    Mouse monoclonal [CD28.2] to CD28, prediluted (PE/Cy7 ®)
  • Host species

    Mouse
  • Conjugation

    PE/Cy7 ®. Ex: 496nm, Em: 774nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Normal Human peripheral blood cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 99% PBS, 0.2% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    CD28.2
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Anti-CD28 antibody [CD28.2], prediluted (Phycoerythrin) (ab134418)
    • Anti-CD28 antibody [CD28.2] (FITC) (ab134472)
    • Anti-CD28 antibody [CD28.2], prediluted (Allophycocyanin) (ab155372)
    • Anti-CD28 antibody [CD28.2], prediluted (PerCP/Cy5.5®) (ab157318)
    • Anti-CD28 antibody [CD28.2] (FITC) (ab234227)
    • Anti-CD28 antibody [CD28.2] (PE/Cy7 ®) (ab234276)
    • Anti-CD28 antibody [CD28.2] (Biotin) (ab267385)
    • Anti-CD28 antibody [CD28.2] - Low endotoxin, Azide free (ab85986)
    • Anti-CD28 antibody [CD28.2] (PerCP) (ab88034)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15H6] (PE/Cy7®) - Isotype control (ab154457)
  • Recombinant Protein

    • Recombinant Human CD28 protein (His tag) (ab222975)

Applications

Our Abpromise guarantee covers the use of ab155387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 106 cells.

ab154457 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
  • Tissue specificity

    Expressed in T-cells and plasma cells, but not in less mature B-cells.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P10747 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 940 Human
    • Omim: 186760 Human
    • SwissProt: P10747 Human
    • Unigene: 591629 Human
    • Alternative names

      • CD 28 antibody
      • CD28 antibody
      • CD28 antigen antibody
      • CD28 molecule antibody
      • CD28_HUMAN antibody
      • MGC138290 antibody
      • T cell antigen CD28 antibody
      • T cell specific surface glycoprotein antibody
      • T cell specific surface glycoprotein CD28 antibody
      • T-cell-specific surface glycoprotein CD28 antibody
      • TP44 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD28 antibody [CD28.2], prediluted (PE/Cy7 ®) (ab155387)
      Flow Cytometry - Anti-CD28 antibody [CD28.2], prediluted (PE/Cy7 ®) (ab155387)
      Flow cytometric analysis of normal Human peripheral blood cells with Anti-Human CD19 FITC and Mouse IgG1 K Isotype Control PE-Cy7 (left) or 5 µl ab155387 (right). Cells in the lymphocyte gate were used for analysis.

    Protocols

    • Flow cytometry protocols

    Datasheets and documents

    • Datasheet
  • References

    ab155387 has not yet been referenced specifically in any publications.

    Publishing research using ab155387? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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