PerCP/Cy5.5® Anti-CD28 antibody [CD28.2], prediluted (ab157318)
Key features and details
- PerCP/Cy5.5® Mouse monoclonal [CD28.2] to CD28, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human, Non human primates
- Conjugation: PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
PerCP/Cy5.5® Anti-CD28 antibody [CD28.2], prediluted
See all CD28 primary antibodies -
Description
PerCP/Cy5.5® Mouse monoclonal [CD28.2] to CD28, prediluted -
Host species
Mouse -
Conjugation
PerCP/Cy5.5®. Ex: 482nm, Em: 690nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human, Non human primates -
Immunogen
DC28.1.3.3 murine T cell hybridoma transfected with Human CD28 cDNA.
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Positive control
- Human blood cells.
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General notes
Purified antibody is conjugated with tandem dye PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading...
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Purity
Size exclusion -
Clonality
Monoclonal -
Clone number
CD28.2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PE Anti-CD28 antibody [CD28.2], prediluted (ab134418)
- FITC Anti-CD28 antibody [CD28.2] (ab134472)
- PE/Cy7® Anti-CD28 antibody [CD28.2] (ab234276)
- Biotin Anti-CD28 antibody [CD28.2] (ab267385)
- APC/Cy7® Anti-CD28 antibody [CD28.2] (ab270676)
- Anti-CD28 antibody [CD28.2] - Low endotoxin, Azide free (ab85986)
- PerCP Anti-CD28 antibody [CD28.2] (ab88034)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 4µl for 106 cells.
4 μl reagent / 100 μl of whole blood. ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 4µl for 106 cells. 4 μl reagent / 100 μl of whole blood. ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
Tissue specificity
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 940 Human
- Omim: 186760 Human
- SwissProt: P10747 Human
- Unigene: 591629 Human
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Alternative names
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab157318 has been referenced in 1 publication.
- Nunes J et al. Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity. Biochem J 293 ( Pt 3):835-42 (1993). PubMed: 8394695