Overview

  • Product name
    Anti-CD28 antibody [CD28.2], prediluted (PerCP/Cy5.5®)
    See all CD28 primary antibodies
  • Description
    Mouse monoclonal [CD28.2] to CD28, prediluted (PerCP/Cy5.5®)
  • Host species
    Mouse
  • Conjugation
    PerCP/Cy5.5®. Ex: 482nm, Em: 690nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Non human primates
  • Immunogen

    DC28.1.3.3 murine T cell hybridoma transfected with Human CD28 cDNA.

  • Positive control
    • Human blood cells.
  • General notes

    Purified antibody is conjugated with tandem dye PerCP/Cy5.5® under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Properties

Applications

Our Abpromise guarantee covers the use of ab157318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 4µl for 106 cells.

(or 100 µl of whole blood.)

 

 

 

ab157226 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
  • Tissue specificity
    Expressed in T-cells and plasma cells, but not in less mature B-cells.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 28 antibody
    • CD28 antibody
    • CD28 antigen antibody
    • CD28 molecule antibody
    • CD28_HUMAN antibody
    • MGC138290 antibody
    • T cell antigen CD28 antibody
    • T cell specific surface glycoprotein antibody
    • T cell specific surface glycoprotein CD28 antibody
    • T-cell-specific surface glycoprotein CD28 antibody
    • TP44 antibody
    see all

References

This product has been referenced in:
  • Nunes J  et al. Signalling through CD28 T-cell activation pathway involves an inositol phospholipid-specific phospholipase C activity. Biochem J 293 ( Pt 3):835-42 (1993). Read more (PubMed: 8394695) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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