Product nameAnti-CD28 antibody [EPR23280-63] - BSA and Azide free
See all CD28 primary antibodies
DescriptionRabbit monoclonal [EPR23280-63] to CD28 - BSA and Azide free
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB
Species reactivityReacts with: Mouse
Recombinant fragment within Mouse CD28 aa 1-150. The exact sequence is proprietary.
Database link: P31041
- Flow cyt: Mouse splenocytes.
ab270034 is the carrier-free version of ab246517. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab270034 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab270034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
FunctionInvolved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
Tissue specificityExpressed in T-cells and plasma cells, but not in less mature B-cells.
Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
Flow cytometric analysis of mouse splenocytes labelling CD28 with ab246517 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG isotype control (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab246517 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246517).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab270034 has not yet been referenced specifically in any publications.