Anti-CD28 antibody [PV-1] (ab25234)

Submit a review Q&A (2)References (6)

Key features and details

  • Armenian hamster monoclonal [PV-1] to CD28
  • Suitable for: IP, Flow Cyt, Functional Studies
  • Reacts with: Mouse
  • Isotype: IgG

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Anti-CD28 antibody [EPR23280-63] (ab246517)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-CD28 antibody [PV-1]
    See all CD28 primary antibodies

  • Description

    Armenian hamster monoclonal [PV-1] to CD28

  • Host species

    Armenian hamster

  • Tested applications

    Suitable for: IP, Flow Cyt, Functional Studiesmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Tissue, cells or virus corresponding to CD28. C57BL/6N mouse T cell lymphoma EL4 cell line

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab25234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

In vitro costimulation of T cells and NK cells: Use at an assay dependent dilution. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
Functional Studies
Use at an assay dependent concentration.

In vitro costimulation of T cells and NK cells.
Notes
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

In vitro costimulation of T cells and NK cells: Use at an assay dependent dilution. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
Functional Studies
Use at an assay dependent concentration.

In vitro costimulation of T cells and NK cells.

Target

  • Function

    Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

  • Tissue specificity

    Expressed in T-cells and plasma cells, but not in less mature B-cells.

  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 28 antibody
      • CD28 antibody
      • CD28 antigen antibody
      • CD28 molecule antibody
      • CD28_HUMAN antibody
      • MGC138290 antibody
      • T cell antigen CD28 antibody
      • T cell specific surface glycoprotein antibody
      • T cell specific surface glycoprotein CD28 antibody
      • T-cell-specific surface glycoprotein CD28 antibody
      • TP44 antibody
      see all

    References (6)

    Publishing research using ab25234? Please let us know so that we can cite the reference in this datasheet.

    ab25234 has been referenced in 6 publications.

    • Bevington SL  et al. Chromatin Priming Renders T Cell Tolerance-Associated Genes Sensitive to Activation below the Signaling Threshold for Immune Response Genes. Cell Rep 31:107748 (2020). PubMed: 32521273
    • Wang J  et al. PDL1 Fusion Protein Protects Against Experimental Cerebral Malaria via Repressing Over-Reactive CD8+ T Cell Responses. Front Immunol 9:3157 (2018). PubMed: 30693001
    • Meyer Zu Horste G  et al. Predicting the Response to Intravenous Immunoglobulins in an Animal Model of Chronic Neuritis. PLoS One 11:e0164099 (2016). Flow Cyt . PubMed: 27711247
    • Mascanfroni ID  et al. Metabolic control of type 1 regulatory T cell differentiation by AHR and HIF1-a. Nat Med 21:638-46 (2015). PubMed: 26005855
    • Fischer N  et al. Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG. Nat Commun 6:6113 (2015). PubMed: 25672245
    • Quintana FJ  et al. Aiolos promotes TH17 differentiation by directly silencing Il2 expression. Nat Immunol 13:770-7 (2012). PubMed: 22751139

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    I thought it would be easier just to ask for help instead of ordering the wrong thing. We're doing a T cell isolation from mouse spleens and need anti-CD3 and anti-CD28 for the culture plates. My boss asked me to get prices for the 500ug size.

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    Answer

    Thank you for contacting us.

    While we do not have any antibodies that are specifically tested for your purposes, I would recommend ab113628 for CD3. This antibody recognizes an extracellular epitope and gives good results in ELISA and Flow Cytometry, which are similar to your assay. For CD28, we currently have two clones that recognize extracellular epitopes and are reactive with mouse: PV-1 (ab25234) and 37.51 (ab25233). These clones are available directly conjugated to a variety of fluorophores and biotin.

    If you will be purchasing more than five vials in total, my colleagues in customer services can help you determine if you are eligible for a discount when you place your order. If you will be purchasing more than 1mg of a single antibody, our bulk sales team can help prepare a quote for you: mailto:sales@abcam.com

    I hope this helps, please let me know if you need any additional information or assistance.

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    Question

    Dear Tech Support Team, Kindly advise if ab25234 is suitable for cell activation assay with mouse cells. Thanks in advance for your assistance and reply. Kind Regards,

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    Answer

    Thank you for contacting us. Clone PV-1 is approved for In vitro costimulation of mouse T cells and NK cells, though we do recommend using the LE/AF format when working with live cells. Product #ab83051 has been tested to ensure low endotoxin levels and is supplied in PBS, pH 7.4. I have added the lnk to ab83051 below: https://www.abcam.com/CD80-antibody-AP-M-Low-endotoxin-Azide-free-ab83051.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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