Anti-CD28 antibody [PV-1] (ab25234)

Submit a review Q&A (2)References (5)

Key features and details

  • Armenian hamster monoclonal [PV-1] to CD28
  • Isotype: IgG

Overview

  • Product name

    Anti-CD28 antibody [PV-1]
    See all CD28 primary antibodies

  • Description

    Armenian hamster monoclonal [PV-1] to CD28

  • Host species

    Armenian hamster

  • Specificity

    ab25234 recognises Murine CD28.

  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Target

  • Function

    Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

  • Tissue specificity

    Expressed in T-cells and plasma cells, but not in less mature B-cells.

  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • CD 28 antibody
    • CD28 antibody
    • CD28 antigen antibody
    • CD28 molecule antibody
    • CD28_HUMAN antibody
    • MGC138290 antibody
    • T cell antigen CD28 antibody
    • T cell specific surface glycoprotein antibody
    • T cell specific surface glycoprotein CD28 antibody
    • T-cell-specific surface glycoprotein CD28 antibody
    • TP44 antibody
    see all

References (5)

Publishing research using ab25234? Please let us know so that we can cite the reference in this datasheet.

ab25234 has been referenced in 5 publications.

  • Wang J  et al. PDL1 Fusion Protein Protects Against Experimental Cerebral Malaria via Repressing Over-Reactive CD8+ T Cell Responses. Front Immunol 9:3157 (2018). PubMed: 30693001
  • Meyer Zu Horste G  et al. Predicting the Response to Intravenous Immunoglobulins in an Animal Model of Chronic Neuritis. PLoS One 11:e0164099 (2016). Flow Cyt . PubMed: 27711247
  • Mascanfroni ID  et al. Metabolic control of type 1 regulatory T cell differentiation by AHR and HIF1-a. Nat Med 21:638-46 (2015). PubMed: 26005855
  • Fischer N  et al. Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG. Nat Commun 6:6113 (2015). PubMed: 25672245
  • Quintana FJ  et al. Aiolos promotes TH17 differentiation by directly silencing Il2 expression. Nat Immunol 13:770-7 (2012). PubMed: 22751139

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

I thought it would be easier just to ask for help instead of ordering the wrong thing. We're doing a T cell isolation from mouse spleens and need anti-CD3 and anti-CD28 for the culture plates. My boss asked me to get prices for the 500ug size.

Read More
Answer

Thank you for contacting us.

While we do not have any antibodies that are specifically tested for your purposes, I would recommend ab113628 for CD3. This antibody recognizes an extracellular epitope and gives good results in ELISA and Flow Cytometry, which are similar to your assay. For CD28, we currently have two clones that recognize extracellular epitopes and are reactive with mouse: PV-1 (ab25234) and 37.51 (ab25233). These clones are available directly conjugated to a variety of fluorophores and biotin.

If you will be purchasing more than five vials in total, my colleagues in customer services can help you determine if you are eligible for a discount when you place your order. If you will be purchasing more than 1mg of a single antibody, our bulk sales team can help prepare a quote for you: mailto:sales@abcam.com

I hope this helps, please let me know if you need any additional information or assistance.

Read More

Question

Dear Tech Support Team, Kindly advise if ab25234 is suitable for cell activation assay with mouse cells. Thanks in advance for your assistance and reply. Kind Regards,

Read More
Answer

Thank you for contacting us. Clone PV-1 is approved for In vitro costimulation of mouse T cells and NK cells, though we do recommend using the LE/AF format when working with live cells. Product #ab83051 has been tested to ensure low endotoxin levels and is supplied in PBS, pH 7.4. I have added the lnk to ab83051 below: https://www.abcam.com/CD80-antibody-AP-M-Low-endotoxin-Azide-free-ab83051.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com