Anti-CD28 antibody [RM0386-17E9] (ab205136)
Key features and details
- Rat monoclonal [RM0386-17E9] to CD28
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2
Overview
-
Product name
Anti-CD28 antibody [RM0386-17E9]
See all CD28 primary antibodies -
Description
Rat monoclonal [RM0386-17E9] to CD28 -
Host species
Rat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat -
Immunogen
Recombinant fragment corresponding to Mouse CD28 aa 21-149.
Sequence:KILVKQSPLL VVDSNEVSLS CRYSYNLLAK EFRASLYKGV NSDVEVCVGN GNFTYQPQFR SNAEFNCDGD FDNETVTFRL WNLHVNHTDI YFCKIEFMYP PPYLDNERSN GTIIHIKEKH LCHTQSSPK
Database link: P31041 -
Positive control
- WB: Mouse thymus tissue lysate.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Lyophilized:Reconstitute the antibody with 500 uL sterile PBS and the final concentration is 200 ug/mL. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
RM0386-17E9 -
Isotype
IgG2 -
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab205136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/2000. Predicted molecular weight: 25 kDa. |
Target
-
Function
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
Tissue specificity
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 12487 Mouse
- Entrez Gene: 25660 Rat
- SwissProt: P31041 Mouse
- SwissProt: P31042 Rat
- Unigene: 255003 Mouse
- Unigene: 10327 Rat
-
Alternative names
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
see all
Images
Datasheets and documents
References (0)
ab205136 has not yet been referenced specifically in any publications.