Anti-CD28 antibody [RM0386-17E9] (ab205136)

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Western blot - Anti-CD28 antibody [RM0386-17E9] (ab205136)

    Key features and details

    • Rat monoclonal [RM0386-17E9] to CD28
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG2

    Overview

    • Product name

      Anti-CD28 antibody [RM0386-17E9]
      See all CD28 primary antibodies

    • Description

      Rat monoclonal [RM0386-17E9] to CD28

    • Host species

      Rat

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Rat

    • Immunogen

      Recombinant fragment corresponding to Mouse CD28 aa 21-149.
      Sequence:

      KILVKQSPLL VVDSNEVSLS CRYSYNLLAK EFRASLYKGV NSDVEVCVGN GNFTYQPQFR SNAEFNCDGD FDNETVTFRL WNLHVNHTDI YFCKIEFMYP PPYLDNERSN GTIIHIKEKH LCHTQSSPK


      Database link: P31041
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • WB: Mouse thymus tissue lysate.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab205136 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/1000 - 1/2000. Predicted molecular weight: 25 kDa.

    Target

    • Function

      Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

    • Tissue specificity

      Expressed in T-cells and plasma cells, but not in less mature B-cells.

    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 28 antibody
        • CD28 antibody
        • CD28 antigen antibody
        • CD28 molecule antibody
        • CD28_HUMAN antibody
        • MGC138290 antibody
        • T cell antigen CD28 antibody
        • T cell specific surface glycoprotein antibody
        • T cell specific surface glycoprotein CD28 antibody
        • T-cell-specific surface glycoprotein CD28 antibody
        • TP44 antibody
        see all

      Images

      • Western blot - Anti-CD28 antibody [RM0386-17E9] (ab205136)
        Western blot - Anti-CD28 antibody [RM0386-17E9] (ab205136)
        Anti-CD28 antibody [RM0386-17E9] (ab205136) at 1/1000 dilution + mouse thymus tissue lysate

        Predicted band size: 25 kDa

      References (0)

      Publishing research using ab205136? Please let us know so that we can cite the reference in this datasheet.

      ab205136 has not yet been referenced specifically in any publications.

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