Overview

  • Product name
    Anti-CD299 antibody [EPR11211]
    See all CD299 primary antibodies
  • Description
    Rabbit monoclonal [EPR11211] to CD299
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Recombinant fragment within Human CD299 aa 200-400. The exact sequence is proprietary.

  • Positive control
    • Human fetal kidney and fetal liver lysates. Human placenta lysate. Human liver and placenta tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11211
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. Is presumably a coreceptor for the SARS coronavirus.
    • Tissue specificity
      Predominantly highly expressed in liver sinusoidal endothelial cells and in lymph node. Found in placental endothelium but not in macrophages. Expressed in type II alveolar cells and lung endothelial cells.
    • Sequence similarities
      Contains 1 C-type lectin domain.
    • Domain
      The tandem repeat domain, also called neck domain, mediates oligomerization.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • C type lectin domain family 4, member M antibody
      • C-type lectin domain family 4 member M antibody
      • CD209 antigen like antibody
      • CD209 antigen like protein 1 antibody
      • CD209 antigen-like protein 1 antibody
      • CD209L antibody
      • CD209L1 antibody
      • CD299 antibody
      • CD299 antigen antibody
      • CLC4M_HUMAN antibody
      • CLEC4M antibody
      • DC SIGN related protein antibody
      • DC SIGN2 antibody
      • DC SIGNR antibody
      • DC-SIGN-related protein antibody
      • DC-SIGN2 antibody
      • DC-SIGNR antibody
      • DCSIGN related protein antibody
      • DCSIGNR antibody
      • Dendritic cell-specific ICAM-3-grabbing non-integrin 2 antibody
      • HP10347 antibody
      • L SIGN antibody
      • L-SIGN antibody
      • Liver/lymph node specific ICAM3 grabbing nonintegrin antibody
      • Liver/lymph node-specific ICAM-3-grabbing non-integrin antibody
      • LSIGN antibody
      • Mannose binding C type lectin DC SIGNR antibody
      • MGC129964 antibody
      • MGC47866 antibody
      see all

    Images

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling CD299 using ab169783 at a 1/100 dilution.
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, labeling CD299 using ab169783 at a 1/100 dilution.
    • All lanes : Anti-CD299 antibody [EPR11211] (ab169783) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 45 kDa

    References

    This product has been referenced in:
    • Wang X  et al. Overexpression of dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin-related protein in cervical cancer and correlation with squamous cell carcinoma antigen. Oncol Lett 14:2813-2821 (2017). Read more (PubMed: 28927040) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab169783.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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