Product nameAnti-CD299 antibody [EPR11211]
See all CD299 primary antibodies
DescriptionRabbit monoclonal [EPR11211] to CD299
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
- Human fetal kidney and fetal liver lysates. Human placenta lysate. Human liver and placenta tissues.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab169783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 45 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionProbable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. Is presumably a coreceptor for the SARS coronavirus.
Tissue specificityPredominantly highly expressed in liver sinusoidal endothelial cells and in lymph node. Found in placental endothelium but not in macrophages. Expressed in type II alveolar cells and lung endothelial cells.
Sequence similaritiesContains 1 C-type lectin domain.
DomainThe tandem repeat domain, also called neck domain, mediates oligomerization.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- C type lectin domain family 4, member M antibody
- C-type lectin domain family 4 member M antibody
- CD209 antigen like antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling CD299 using ab169783 at a 1/100 dilution.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, labeling CD299 using ab169783 at a 1/100 dilution.
All lanes : Anti-CD299 antibody [EPR11211] (ab169783) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 45 kDa
This product has been referenced in:
- Wang X et al. Overexpression of dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin-related protein in cervical cancer and correlation with squamous cell carcinoma antigen. Oncol Lett 14:2813-2821 (2017). Read more (PubMed: 28927040) »