Overview

  • Product name

  • Description

    Rabbit polyclonal to CD2AP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human CD2AP. The exact sequence is proprietary. NP_036252.1.
    Database link: Q9Y5K6

  • Positive control

    • HeLa, Jurkat and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab205017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 71 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis.
  • Tissue specificity

    Widely expressed in fetal and adult tissues.
  • Involvement in disease

    Defects in CD2AP are the cause of susceptibility to focal segmental glomerulosclerosis type 3 (FSGS3) [MIM:607832]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similarities

    Contains 3 SH3 domains.
  • Domain

    The Pro-rich domain may mediate binding to SH3 domains.
    Potential homodimerization is mediated by the coiled coil domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues; probably by c-Abl, Fyn and c-Src.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cell projection > ruffle. Colocalizes with F-actin and BCAR1/p130Cas in membrane ruffles. Located at podocyte slit diaphragm between podocyte foot processes (By similarity). During late anaphase and telophase, concentrates in the vicinity of the midzone microtubules and in the midbody in late telophase.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adapter protein CMS antibody
    • AL024079 antibody
    • C78928 antibody
    • Cas ligand with multiple SH3 domains antibody
    • CD2 associated protein antibody
    • CD2-associated protein antibody
    • CD2AP antibody
    • CD2AP_HUMAN antibody
    • CMS antibody
    • Mesenchyme to epithelium transition protein with SH3 domains 1 antibody
    • METS 1 antibody
    • Mets1 antibody
    see all

Images

  • All lanes : Anti-CD2AP antibody (ab205017) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 71 kDa


    Exposure time: 30 seconds
  • Detection of CD2AP in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205017 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab205017 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab205017 has not yet been referenced specifically in any publications.

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