Key features and details
- Rabbit polyclonal to CD2AP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CD2AP antibody
See all CD2AP primary antibodies
DescriptionRabbit polyclonal to CD2AP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Recombinant fragment within Human CD2AP. The exact sequence is proprietary. NP_036252.1.
Database link: Q9Y5K6
- HeLa, Jurkat and 293T whole cell lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab205017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 71 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionSeems to act as an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis.
Tissue specificityWidely expressed in fetal and adult tissues.
Involvement in diseaseDefects in CD2AP are the cause of susceptibility to focal segmental glomerulosclerosis type 3 (FSGS3) [MIM:607832]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
Sequence similaritiesContains 3 SH3 domains.
DomainThe Pro-rich domain may mediate binding to SH3 domains.
Potential homodimerization is mediated by the coiled coil domain.
modificationsPhosphorylated on tyrosine residues; probably by c-Abl, Fyn and c-Src.
Cellular localizationCytoplasm > cytoskeleton. Cell projection > ruffle. Colocalizes with F-actin and BCAR1/p130Cas in membrane ruffles. Located at podocyte slit diaphragm between podocyte foot processes (By similarity). During late anaphase and telophase, concentrates in the vicinity of the midzone microtubules and in the midbody in late telophase.
- Information by UniProt
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All lanes : Anti-CD2AP antibody (ab205017) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 71 kDa
Exposure time: 30 seconds
Detection of CD2AP in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab205017 at 6 µg/mg lysate for IP (Lane 1). For WB detection an ab205017 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
ab205017 has been referenced in 1 publication.
- Thuault S et al. A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cells. Sci Rep 10:6787 (2020). PubMed: 32321993