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    cd2bp2-antibody-ab241947.pdf

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Immunology Adaptive Immunity T Cells CD
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Anti-CD2BP2 antibody (ab241947)

  • Datasheet
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Abpromise

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Western blot - Anti-CD2BP2 antibody (ab241947)

    Key features and details

    • Rabbit polyclonal to CD2BP2
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-CD2BP2 antibody
      See all CD2BP2 primary antibodies
    • Description

      Rabbit polyclonal to CD2BP2
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse, Human
    • Immunogen

      Synthetic peptide within Human CD2BP2 aa 275-325. The exact sequence is proprietary.
      Database link: O95400

    • Positive control

      • WB: HeLa, HEK-239T, TCMK-1 and NIH/3T3 whole cell lysate.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: Tris citrate/phosphate

      pH 7 to 8
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Antibody was affinity purified using an epitope specific to CD2BP2 immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Immunology
      • Immune System Diseases
      • Antiviral Signaling

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • HeLa whole cell lysate (ab150035)
      • HeLa whole cell lysate (ab29545)
      • NIH 3T3 whole cell lysate (ab7179)

    Applications

    Our Abpromise guarantee covers the use of ab241947 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/1000 - 1/5000.

    Target

    • Function

      Involved in pre-mRNA splicing as component of the U5 snRNP complex that is involved in spliceosome assembly.
    • Sequence similarities

      Contains 1 GYF domain.
    • Cellular localization

      Cytoplasm. Nucleus. Predominantly nuclear.
    • Target information above from: UniProt accession O95400 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10421 Human
      • Entrez Gene: 70233 Mouse
      • Omim: 604470 Human
      • SwissProt: O95400 Human
      • SwissProt: Q9CWK3 Mouse
      • Unigene: 202677 Human
      • Unigene: 28050 Mouse
      • Alternative names

        • CD2 (cytoplasmic tail) binding protein 2 antibody
        • CD2 antigen (cytoplasmic tail) binding protein 2 antibody
        • CD2 antigen cytoplasmic tail-binding protein 2 antibody
        • CD2 cytoplasmic domain-binding protein 2 antibody
        • CD2 tail-binding protein 2 antibody
        • CD2B2_HUMAN antibody
        • Cd2bp2 antibody
        • FWP010 antibody
        • LIN1 antibody
        • Snu40 antibody
        • U5 snRNP 52K protein antibody
        • U5-52K antibody
        see all

      Images

      • Western blot - Anti-CD2BP2 antibody (ab241947)
        Western blot - Anti-CD2BP2 antibody (ab241947)
        All lanes : Anti-CD2BP2 antibody (ab241947) at 0.4 µg/ml

        Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
        Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
        Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
        Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
        Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

        Lysates/proteins at 50 µg per lane.

        Exposure time: 3 minutes


        Prepared using NETN lysis buffer.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab241947? Please let us know so that we can cite the reference in this datasheet.

      ab241947 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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