Anti-CD3 antibody (ab5690)

Thank you for confirming the details.

This antibody has been positively used in many publications and in more than 25 positive Abreviews so I am sorry we are not sure the cause of the problem.

Also we have not tested this antibody with free floating Brain tissue sections so we are not aware about any non specificity. This antibody has been successfully used in free floating sections in the publication http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=20126467&dopt=Abstract. We ourselves do not hold any IHC-FoFr application data.

Interestingly in the following publication http://www.molbiolcell.org/content/19/6/2444.full.pdf Fig 2G the anti CD3 antibody seems staining the axonal processes. Please check.

Finally, I would like to suggest optimizing the protocol e.g. try changing the conc./dilution of primary antibody, try no primary control

I am sure with minor protocol tweaks the antibody would give you expected staining. If however the results do not improve, please do not hesitate to contact me for further assistance.

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

- Could you explain the probe conjugated secondary antibody? What color of the pobe should be?
- Did you fixed the sections in acetone or PFA after putting these on slide?
- Please provide the purhcase order number and details about conditons of antibody storage?

Thank you very much for your cooperation. I will look forward to hearing from you soon.

Thank you for contacting Abcam.
I am sorry about the antibody is covered under our Abpromise for six months and is guaranteed to work in IHC-P on samples . If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.
To be able to help further in this matter, would you please be able to provide some images of the staining pattern you are receiving ab5690?
I look forward to your reply and helping you resolve this issue.

Thank you for contacting us.

As stated on the datasheet, this antibody is not conjugated and has been used in combination with some HRP- conjugated secondary antibodies in our IHC experiments (see images).

As a Biotin-conjugated secondary I would suggest

https://www.abcam.com/Goat-Fab2-Rabbit-IgG-HL-Biotin-pre-adsorbed-ab98506.html (or use the following: https://www.abcam.com/Goat-Fab2-Rabbit-IgG-HL-Biotin-pre-adsorbed-ab98506.html).

This antibody is pre-adsorbed for minimal cross-reactivity and comes as F(ab)2 fragments, which should nicely amplify signal, too.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting Abcam regarding ab5690.


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.


I wish you the best of luck with your research.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ######.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our 6 month Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you very much for your call today.

Sinceab4055 has not been tested with mouse samples, I can offer a discount off a future purchase if you buy ab4055 now, test it with mouse samples and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would beworth 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab4055 with mouse samples. I will then send a discount code. This code must be issued before purchasing ab4055 so please wait for my reply before ordering.

2. Purchase ab4055 either by phone, fax, or online (www.abcam.com).

3. Test it with mouse samples.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab4055 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Thank you for your phone call. This antibody has not yet been tested in flow cytometry. Therefore, we can offer you a testing discount code for trying it in flow cytometry. For detailed instructions, please see below.

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab5690 in flow cytometry. This code will give you: 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for flow cytometryand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.