Anti-CD3 antibody (ab828)
Key features and details
- Rabbit polyclonal to CD3
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD3 antibody
See all CD3 primary antibodies -
Description
Rabbit polyclonal to CD3 -
Host species
Rabbit -
Specificity
ab828 is predicted to react with T cell areas in lymphoid tissues derived from various species: human, bovine, porcine, sheep, cat, dog, rat, mouse, Psammomys (sand rat), and chicken. The molecular weight of the polypeptide recognized by the antibody in bovine and swine extracts is 20 kDa.
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Tested applications
Suitable for: IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human CD3 conjugated to Bovine Serum Albumin (BSA).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (8) |
Use at an assay dependent concentration.
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IHC-Fr |
1/10 - 1/50. ABC method.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
IHC-Fr
1/10 - 1/50. ABC method. |
Images
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This immunoperoxidase picture shows inflammatory cell infiltration with many T-lymphocytes in the interstitium of human kidneys with congenital nephrotic syndrome of the Finnish type (CNF, NPHS1). Immunoperoxidase staining of frozen sections with ab828 antibody (oval shaped, unstained spaces on the left are glomeruli).
This image was kindly supplied as part of the review submitted by Arvi-Matti-Kuusniemi. -
Ab828 positively staining formaldehyde fixed human tonsil tissue at 1/50 in conjunction with goat anti rabbit HRP. Detection by DAB.
Pressure cooker heat mediated antigen retrieval was employed in citrate buffer pH 6.0.
This image is courtesy of an Abreview submitted by Melanie Black on 22 Aug 05. We do not have any further information relating to this image.
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IHC-P image of CD3 staining on rat small intestine using ab828 (1:50). The section was deparafinized, rehydrated and subjected to heat mediated antigen retrival using citric acid at pH6. The section was then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 21°C for 2 hours. Goat polyclonal to Rabbit IgG conjugated to biotin was used as secondary antibody (1:250).
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IHC-P image of CD3 staining on monkey colon using ab828 (1:50). The section was deparafinized, rehydrated and subjected to heat mediated antigen retrival using citric acid at pH6. The section was then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 21°C for 2 hours. Goat polyclonal to Rabbit IgG conjugated to biotin was used as secondary antibody (1:250).
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IHC-P image of CD3 staining on dog spleen using ab828 (1:50). The section was deparafinized, rehydrated and subjected to heat mediated antigen retrival using citric acid at pH6. The section was then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 21°C for 2 hours. Goat polyclonal to Rabbit IgG conjugated to biotin was used as secondary antibody (1:250).
Datasheets and documents
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SDS download
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Datasheet download
References (47)
ab828 has been referenced in 47 publications.
- Köseer AS et al. Validation of CD98hc as a Therapeutic Target for a Combination of Radiation and Immunotherapies in Head and Neck Squamous Cell Carcinoma. Cancers (Basel) 14:N/A (2022). PubMed: 35406454
- Mokhtar DM et al. Ependymal and Neural Stem Cells of Adult Molly Fish (Poecilia sphenops, Valenciennes, 1846) Brain: Histomorphometry, Immunohistochemical, and Ultrastructural Studies. Cells 11:N/A (2022). PubMed: 36078068
- Mokhtar DM The structural and ultrastructural organization of the cellular constituents of the trunk kidney of grass carp (Ctenopharyngodon idella). Microsc Res Tech 84:537-547 (2021). PubMed: 32986903
- Ramirez M et al. Semi-automated digital quantification of cellular infiltrates for in vivo evaluation of transplanted islets of Langerhans encapsulated with bioactive materials. Xenotransplantation 28:e12704 (2021). PubMed: 34218466
- Mori A et al. Establishment of Human Leukocyte Antigen-Mismatched Immune Responses after Transplantation of Human Liver Bud in Humanized Mouse Models. Cells 10:N/A (2021). PubMed: 33672150