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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TRIS/BSA/azide
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
A disappointing dilution factor.
However, using a more sensitiveroutine detection system ( such as a Micropolymer kit) will increase the factor 2-4 times.
Mr. Carl Hobbs
Submitted Sep 07 2012