Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Specification
Tonsil
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution
1/25
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TRIS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250

Additional data

Additional Notes
A disappointing dilution factor.
However, using a more sensitiveroutine detection system ( such as a Micropolymer kit) will increase the factor 2-4 times.

Mr. Carl Hobbs

Verified customer

Submitted Sep 07 2012

Sign up