Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Small intestine)
Specification
Small intestine
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution
1/50
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250

Additional data

Additional Notes
Interestingly, on rat, this Ab gives a crisp clean positivity
However, the dilution factor is poor for a rabbit polyclonal.
Note that the tips of the villi show poor preservation of the lining epithelium this is due to poor fixation.
Gut should be not be immersion-fixed without prior dissection and cleaning of contents.

Mr. Carl Hobbs

Verified customer

Submitted Oct 04 2012

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