Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody

Poor
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution
1/50
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA.azide

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250

Additional data

Additional Notes
Submitted composite image shows that this Ab is unsuitable for cat, in my hands: ( upper image is Abcam Ab, lower image of another anti CD3 Ab).
Abcam response
Thank you for your valuable feedback. ab828 had not been previously tested in cat tissue and your data suggests that ab828 may not detect CD3 on cat tissue under these experimental conditions. We hope this information is useful to our customers.

Mr. Carl Hobbs

Verified customer

Submitted Oct 04 2012

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