BSA as blocking agent for 30 minute(s) · Concentration: 1%
Other product details
Non-Abcam antibody was used: DAKO Envision Anti Rabbit Host species: Goat Clonality: Monoclonal Conjugation: Horse Radish Peroxidase
I have found this antibody to work well in human and rabbit tissue at a dilution of 1:50 with pressure cooking in citrate buffer pH 6, for antigen retrieval. My secondary antibody was DAKO Envision anti-Rabbit, followed by DAB. I also had success with Fluorescent staining using Cy 3 as a secondary, using the same dilution.
Mrs. Melanie Black
Submitted Aug 22 2005
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