Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Purified protein (Tonsil)
Specification
Tonsil
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1%

Other product details

Dilution
1/50
Incubation time
45 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: DAKO Envision Anti Rabbit
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
I have found this antibody to work well in human and rabbit tissue at a dilution of 1:50 with pressure cooking in citrate buffer pH 6, for antigen retrieval. My secondary antibody was DAKO Envision anti-Rabbit, followed by DAB. I also had success with Fluorescent staining using Cy 3 as a secondary, using the same dilution.

Mrs. Melanie Black

Verified customer

Submitted Aug 22 2005

Sign up