Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Cell (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 95°C antigen retrieval 20 min in low pH DAKO target retrieval solution S1700
Permeabilization
Yes - TBS with 0.025% Triton
Specification
spleen
Blocking step
TBS with 2%donkey serum and 2%BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS with 2%donkey serum and 2%BSA
Dilution
1/50

Secondary antibody

Dilution
1/100
Name
Non-Abcam antibody was used: donkey antirabbit Alexa 488 (Jackson 711-545-152)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
This human-specific antibody is cross reactive with mouse. Also successfully stained human kidney under same conditions. Antibody applied to PFA-fixed, parafin embedded tissues following deparafinization in Xylol and decreasing Ethanol concentrations. DAKO MAX block reagent A applied after 70% Ethanol. Then 95°C antigen retrieval 20 min in low pH DAKO target retrieval solution S1700 before blocking step.
Abcam response
Thank you for participating in the AbTrial program and your valuable feedback. We are pleased that this antibody worked well for you in mouse, a species that has not been tested before. We welcome any further feedback and AbReviews from other customers who have used this antibody in mouse which will help us assess how well the antibody works in this species.

Mrs. Meaghan Stolk

Verified customer

Submitted May 09 2016

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