Overview

  • Product name
    Anti-CD3 antibody [B355.1 (RIV-9)]
    See all CD3 primary antibodies
  • Description
    Mouse monoclonal [B355.1 (RIV-9)] to CD3
  • Host species
    Mouse
  • Specificity
    This antibody reacts with 5 invariable CD3 chains: CD3y or gp26 CD3d or gp20 CD3e or gp20 CD3f or p16 (homodimer) CD3n or p28 This antibody reacts mainly with T-cells including thymocytes, mature T-cells and T-cell lines
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Human peripheral lymphocytes.

  • Positive control
    • Tonsil, thymus or spleen.

Properties

Applications

Our Abpromise guarantee covers the use of ab8671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1-5µg for 106 cells.

This product was not quality controlled in flow cytometry

 

 

 

ab91537 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use a concentration of 5 - 20 µg/ml. Fix with acetone and use an avidin/biotin system.
WB Use a concentration of 1 - 5 µg/ml.

Target

  • Function
    The CD3 complex mediates signal transduction.
  • Involvement in disease
    Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • Sequence similarities
    Contains 1 ITAM domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • Cd247 antibody
    • CD247 antigen antibody
    • CD247 antigen, zeta subunit antibody
    • CD247 molecule antibody
    • CD3 antibody
    • CD3 antigen, delta subunit antibody
    • CD3 delta antibody
    • CD3 epsilon antibody
    • CD3 eta antibody
    • CD3 gamma antibody
    • CD3 molecule delta polypeptide antibody
    • CD3 molecule, epsilon polypeptide antibody
    • CD3 molecule, gamma polypeptide antibody
    • CD3 zeta antibody
    • CD3-DELTA antibody
    • CD3d antibody
    • CD3D antigen delta polypeptide antibody
    • CD3d antigen, delta polypeptide (TiT3 complex) antibody
    • CD3d molecule delta antibody
    • CD3d molecule delta CD3 TCR complex antibody
    • CD3d molecule, delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • CD3E antibody
    • CD3e antigen antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule epsilon CD3 TCR complex antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3epsilon antibody
    • CD3G antibody
    • CD3g antigen antibody
    • CD3G antigen gamma polypeptide antibody
    • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
    • CD3g molecule gamma antibody
    • CD3g molecule gamma CD3 TCR complex antibody
    • CD3g molecule, gamma (CD3-TCR complex) antibody
    • CD3H antibody
    • CD3Q antibody
    • CD3Z antibody
    • CD3zeta antibody
    • Ctg3 antibody
    • FLJ17620 antibody
    • FLJ17664 antibody
    • FLJ18683 antibody
    • FLJ79544 antibody
    • FLJ94613 antibody
    • IMD19 antibody
    • Leu-4 antibody
    • MGC138597 antibody
    • OKT3, delta chain antibody
    • OTTHUMP00000032544 antibody
    • T cell receptor antibody
    • T cell receptor T3 delta chain antibody
    • T cell receptor T3 gamma chain antibody
    • T cell receptor T3 zeta chain antibody
    • T cell receptor zeta chain antibody
    • T cell surface glycoprotein CD3 antibody
    • T cell surface glycoprotein CD3 delta chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T cell surface glycoprotein CD3 gamma chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, delta subunit of T3 antibody
    • T-cell antigen receptor complex, epsilon subunit of T3 antibody
    • T-cell antigen receptor complex, gamma subunit of T3 antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell receptor T3 gamma chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T-cell surface glycoprotein CD3 gamma chain antibody
    • T3 antibody
    • T3d antibody
    • T3e antibody
    • T3g antibody
    • T3z antibody
    • TCRE antibody
    • TCRk antibody
    • Tcrz antibody
    • TCRzeta antibody
    see all

References

This product has been referenced in:
  • Halum SL  et al. Stem cell-derived tissue-engineered constructs for hemilaryngeal reconstruction. Ann Otol Rhinol Laryngol 123:124-34 (2014). Rat . Read more (PubMed: 24574468) »
  • Johnson G  et al. In vitro assessment of blood compatibility: residual and dynamic markers of cellular activation. J Biomater Appl 27:925-36 (2013). Human . Read more (PubMed: 22210807) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

We currently do not offer products which meet each of the requirements that you have set. I assembled for you well characterized products which meet most of these requirements but each has some deficiency. Pricing is to be found near the top of the webpage for each product directly below the bold type face product name. There is not currently any special pricing at JHMI. Each datasheet contains all images which we currently have for the products. Any publications which we are aware of the product being used in are located behind the specific references tab or at the bottom of the page.

I would recommend rabbit antibodies as it appears you want to test in mouse however I have added mouse antibodies should you wish to use a mouse on mouse IHC kit such as theMouse on Mouse (M.O.M) Polymer IHC Kit ab127055:https://www.abcam.com/Mouse-on-Mouse-MOM-Polymer-IHC-Kit-ab127055.html



Unconjugated anti-CD3 that targets rat, mouse and pig produced in any animal:

ab16669; rabbit monoclonal; predicted based on sequence homology but not yet tested in Pig

https://www.abcam.com/CD3-antibody-SP7-ab16669.html

Or

ab8671; Mouse monoclonal; predicted but not tested in Pig based on sequence homology; not yet tested in IHC-P

https://www.abcam.com/CD3-antibody-B355-1-RIV-9-ab8671.html



Unconjugated anti-CD4 that targets rat, mouse and pig produced in that same animal:

Ab61131; Rabbit polyclonal; predicted but not tested in Pig, Mouse, Rat based on sequence homology

https://www.abcam.com/CD4-antibody-ab61131.html

Or

ab125711; Mouse monoclonal;predicted in pig; not yet tested in mouse

https://www.abcam.com/CD4-antibody-10B5-ab125711.html



Unconjugated anti-CD8 that targets rat, mouse and pig produced in that same animal:

Ab4055; Rabbit polyclonal, predicted but not tested in Pig, Mouse, Rat based on sequence homology

https://www.abcam.com/CD8-antibody-ab4055.html

Or

Ab17147; Mouse monoclonal, not yet tested in Mouse, Rat or Pig. I do not that the exact immunogen however the C-terminal end is well conserved in each species as such we predict this product may work in these species

https://www.abcam.com/CD8-antibody-144B-ab17147.html



Unconjugated anti-FOXP3 that targets rat, mouse and pig produced in that same animal:

ab75763; Rabbit polyclonal; not yet tested in rat and pig in IHC-P This product is the only rabbit host which is predicted to work in all these species;

https://www.abcam.com/FOXP3-antibody-ab75763.html

Or

ab22510; Mouse monoclonal; predicted but not yet tested in mouse and pig based on sequence homology

https://www.abcam.com/FOXP3-antibody-mAbcam-22510-ab22510.html



Horseradish peroxidase-conjugated anti-that same animal IgG:

Ab97051; Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-HRP-ab97051.html

Or

Ab97023; Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP)

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-HL-HRP-ab97023.html



For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

We do not know whether ab8671 will activate T cells to produce IL2 when co stimulated with PMA. The only information we have is that this antibody induces T-cell activation and proliferation

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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