Product nameAnti-CD3 antibody [BL-298-5D12]
See all CD3 epsilon primary antibodies
DescriptionRabbit monoclonal [BL-298-5D12] to CD3
Tested applicationsSuitable for: WB, IHC-P, ICC, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CD3 aa 157-207. The exact sequence is proprietary. NP_000724.1 and Gene ID 916.
Database link: P07766
This product is sold under License from Bethyl Laboratories, Inc.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/100 - 1/500.|
|IP||Use at an assay dependent concentration.
Use 20µl/mg lysate.
FunctionThe CD3 complex mediates signal transduction.
Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain.
- Information by UniProt
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
CD3E was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab243873 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243873 at 1/1000 dilution.
Lane 1: ab243873 IP in Jurkat whole cell lysate.
Lane 2: Contol IgG in Jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Western blot analysis using ab243873 at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate (50 µg).
Lane 2: MOLT-4 whole cell lysate (50 µg).
Lane 3: K-562 whole cell lysate (50 µg).
Lane 4: SK whole cell lysate (50 µg).
Lane 5: HeLa whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 1 seconds
Formaldehyde-fixed MOLT-4 (human lymphoblastic leukemia cell line) cells labeling CD3E using ab243873 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD3E using ab243873 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
ab243873 has not yet been referenced specifically in any publications.