Recombinant

Recombinant Anti-CD3 antibody [BL-298-5D12] (ab243873)

Overview

  • Product name

    Anti-CD3 antibody [BL-298-5D12]
    See all CD3 epsilon primary antibodies
  • Description

    Rabbit monoclonal [BL-298-5D12] to CD3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD3 aa 157-207. The exact sequence is proprietary. NP_000724.1 and Gene ID 916.
    Database link: P07766

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab243873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/500.
IP Use at an assay dependent concentration.

Use 20µl/mg lysate.

Target

  • Function

    The CD3 complex mediates signal transduction.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

Images

  • CD3E was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab243873 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243873 at 1/1000 dilution.

    Lane 1: ab243873 IP in Jurkat whole cell lysate.

    Lane 2: Contol IgG in Jurkat whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

  • Western blot analysis using ab243873 at 1/1000 dilution.

    Lane 1: Jurkat whole cell lysate (50 µg).

    Lane 2: MOLT-4 whole cell lysate (50 µg).

    Lane 3: K-562 whole cell lysate (50 µg).

    Lane 4: SK whole cell lysate (50 µg).

    Lane 5: HeLa whole cell lysate (50 µg).

    A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 1 seconds

  • Formaldehyde-fixed MOLT-4 (human lymphoblastic leukemia cell line) cells labeling CD3E using ab243873 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining
  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD3E using ab243873 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

References

ab243873 has not yet been referenced specifically in any publications.

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