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  1. Link

    cd3-antibody-cd3-12-ab11089.pdf

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Immunology Adaptive Immunity T Cells CD
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Anti-CD3 antibody [CD3-12] (ab11089)

  • Datasheet
  • SDS
Reviews (17)Q&A (3)References (58)

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Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (ab11089)
  • Western blot - Anti-CD3 antibody [CD3-12] (ab11089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)
  • Western blot - Anti-CD3 antibody [CD3-12] (ab11089)
  • Western blot - Anti-CD3 antibody [CD3-12] (ab11089)
  • Western blot - Anti-CD3 antibody [CD3-12] (ab11089)

Key features and details

  • Rat monoclonal [CD3-12] to CD3
  • Suitable for: IHC-P, WB, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-CD3 antibody [CD3-12]
    See all CD3 primary antibodies
  • Description

    Rat monoclonal [CD3-12] to CD3
  • Host species

    Rat
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig, Duck, Cynomolgus monkey, Rhesus monkey, Woodchuck, Raccoon, Alpaca
  • Immunogen

    Synthetic peptide corresponding to Human CD3. Derived from cytoplasmic epitope.
    Database link: P07766

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human tonsil tissue. Mouse spleen and lymph node tissue; Rat spleen tissue. Flow Cyt (Intra): Human peripheral blood lymphocytes. WB: Jurkat, MOLT-4 and EL4 cell lysates; Human, mouse and rat thymus tissue lysates.
  • General notes

    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified IgG prepared from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    CD3-12
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

Associated products

  • Alternative Versions

    • Anti-CD3 antibody [CD3-12] - BSA and Azide free (ab255972)
    • FITC Anti-CD3 antibody [CD3-12] (ab34722)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rat IgG1, kappa monoclonal [RTK2071] - Isotype control (ab18412)
  • Recombinant Protein

    • Recombinant Mouse CD3 protein (Fc Chimera) (ab220543)
    • Recombinant Human CD3 epsilon protein (Fc Chimera His Tag) (ab220590)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Human CD3 peptide (ab16206)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (14)
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

1mM EDTA pH8.0 is recommended for this purpose.

WB
Use at an assay dependent concentration. Predicted molecular weight: 23.1 kDa.
Flow Cyt (Intra)
1/50 - 1/100.

Membrane permeabilization is recommended for this application. ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Notes
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

1mM EDTA pH8.0 is recommended for this purpose.

WB
Use at an assay dependent concentration. Predicted molecular weight: 23.1 kDa.
Flow Cyt (Intra)
1/50 - 1/100.

Membrane permeabilization is recommended for this application. ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    The CD3 complex mediates signal transduction.
  • Involvement in disease

    Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • Sequence similarities

    Contains 1 ITAM domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P04234 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 915 Human
    • Entrez Gene: 916 Human
    • Entrez Gene: 917 Human
    • Entrez Gene: 919 Human
    • Entrez Gene: 12500 Mouse
    • Entrez Gene: 12501 Mouse
    • Entrez Gene: 12502 Mouse
    • Entrez Gene: 12503 Mouse
    • Entrez Gene: 25300 Rat
    • Entrez Gene: 25710 Rat
    • Entrez Gene: 300678 Rat
    • Entrez Gene: 315609 Rat
    • Omim: 186740 Human
    • Omim: 186780 Human
    • Omim: 186790 Human
    • Omim: 186830 Human
    • SwissProt: P04234 Human
    • SwissProt: P07766 Human
    • SwissProt: P09693 Human
    • SwissProt: P20963 Human
    • SwissProt: P04235 Mouse
    • SwissProt: P11942 Mouse
    • SwissProt: P22646 Mouse
    • SwissProt: P24161 Mouse
    • SwissProt: P29020 Mouse
    • SwissProt: P19377 Rat
    • SwissProt: Q64159 Rat
    • Unigene: 504048 Human
    • Unigene: 4527 Mouse
    • Unigene: 10329 Rat
    see all
  • Alternative names

    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • Cd247 antibody
    • CD247 antigen antibody
    • CD247 antigen, zeta subunit antibody
    • CD247 molecule antibody
    • CD3 antibody
    • CD3 antigen, delta subunit antibody
    • CD3 delta antibody
    • CD3 epsilon antibody
    • CD3 eta antibody
    • CD3 gamma antibody
    • CD3 molecule delta polypeptide antibody
    • CD3 molecule, epsilon polypeptide antibody
    • CD3 molecule, gamma polypeptide antibody
    • CD3 zeta antibody
    • CD3-DELTA antibody
    • CD3d antibody
    • CD3D antigen delta polypeptide antibody
    • CD3d antigen, delta polypeptide (TiT3 complex) antibody
    • CD3d molecule delta antibody
    • CD3d molecule delta CD3 TCR complex antibody
    • CD3d molecule, delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • CD3E antibody
    • CD3e antigen antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule epsilon CD3 TCR complex antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3epsilon antibody
    • CD3G antibody
    • CD3g antigen antibody
    • CD3G antigen gamma polypeptide antibody
    • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
    • CD3g molecule gamma antibody
    • CD3g molecule gamma CD3 TCR complex antibody
    • CD3g molecule, gamma (CD3-TCR complex) antibody
    • CD3H antibody
    • CD3Q antibody
    • CD3Z antibody
    • CD3zeta antibody
    • Ctg3 antibody
    • FLJ17620 antibody
    • FLJ17664 antibody
    • FLJ18683 antibody
    • FLJ79544 antibody
    • FLJ94613 antibody
    • IMD19 antibody
    • Leu-4 antibody
    • MGC138597 antibody
    • OKT3, delta chain antibody
    • OTTHUMP00000032544 antibody
    • T cell receptor antibody
    • T cell receptor T3 delta chain antibody
    • T cell receptor T3 gamma chain antibody
    • T cell receptor T3 zeta chain antibody
    • T cell receptor zeta chain antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 antibody
    • T cell surface glycoprotein CD3 delta chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T cell surface glycoprotein CD3 gamma chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, delta subunit of T3 antibody
    • T-cell antigen receptor complex, epsilon subunit of T3 antibody
    • T-cell antigen receptor complex, gamma subunit of T3 antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell receptor T3 gamma chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface glycoprotein CD3 gamma chain antibody
    • T3 antibody
    • T3d antibody
    • T3e antibody
    • T3g antibody
    • T3z antibody
    • TCRE antibody
    • TCRk antibody
    • Tcrz antibody
    • TCRzeta antibody
    see all

Images

  • Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (ab11089)
    Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (ab11089)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized human peripheral blood mononuclear cells (PBMC) labeling CD3 with ab11089 at 1/400 dilution (red) compared with a Mouse IgG, monoclonal Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG ab150157 (Alexa Fluor®488) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Western blot - Anti-CD3 antibody [CD3-12] (ab11089)
    Western blot - Anti-CD3 antibody [CD3-12] (ab11089)
    All lanes : Anti-CD3 antibody [CD3-12] (ab11089) at 1 µg/ml

    Lane 1 : THP-1 (Human monocytic leukemia cell line) whole cell lysate (negative control)
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate (negative control)
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : Human thymus tissue lysate
    Lane 5 : Mouse thymus tissue lysate
    Lane 6 : Rat thymus tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-Rat at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 23.1 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?



    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab11089 overnight at 4°C. Antibody binding was detected using Goat anti-Rat secondary at a 1:10000 dilution for 1hr at room temperature and then imaged.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CB3 with ab11089 at 1/200 dilution, followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on rat spleen tissue is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD3 with ab11089 at 1/200 dilution, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on mouse spleen tissue is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)

    IHC image of CD3 staining in a formalin fixed, paraffin embedded human tonsil tissue. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6). The section was incubated with ab11089 at 1/250 dilution for 15 minutes at room temperature. A goat anti-rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)

    IHC image of CD3 staining in a formalin fixed, paraffin embedded normal mouse lymph node tissue.

    The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6). The section was incubated with ab11089 at 1/250 dilution for 15 minutes at room temperature. A goat anti-rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)This image is courtesy of an anonymous Abreview.

    ab11089 staining CD3 in mouse lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with formaldehyde and blocked with 15% serum for 60 minutes at 20°C. Antigen retrieval was by heat mediation in sodium citrate (pH 6). Samples were incubated with primary antibody (1/250 in TBS) for 18 hours at 20°C. An Alexa Fluor®conjugated goat anti-rat IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-CD3 antibody [CD3-12] (ab11089)
    Western blot - Anti-CD3 antibody [CD3-12] (ab11089)
    All lanes : Anti-CD3 antibody [CD3-12] (ab11089) at 1/1000 dilution

    Lane 1 : Mouse thymus tissue lysate
    Lane 2 : Rat thymus tissue lysate
    Lane 3 : Human thymus tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 23.1 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure: Lanes 1/3: 5 secs; Lanes 2: 3 secs.

  • Western blot - Anti-CD3 antibody [CD3-12] (ab11089)
    Western blot - Anti-CD3 antibody [CD3-12] (ab11089)
    All lanes : Anti-CD3 antibody [CD3-12] (ab11089) at 1/1000 dilution

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte)
    Lane 2 : MOLT-4 (human lymphoblastic leukemia T lymphoblast)
    Lane 3 : EL4 (mouse lymphoma T lymphocyte)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 23.1 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 5 secs.

  • Western blot - Anti-CD3 antibody [CD3-12] (ab11089)
    Western blot - Anti-CD3 antibody [CD3-12] (ab11089)
    Anti-CD3 antibody [CD3-12] (ab11089) + Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Secondary
    HRP-conjugated goat anti-rat IgG

    Predicted band size: 23.1 kDa

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (58)

Publishing research using ab11089? Please let us know so that we can cite the reference in this datasheet.

ab11089 has been referenced in 58 publications.

  • Gernoux G  et al. AAV8 locoregional delivery induces long-term expression of an immunogenic transgene in macaques despite persisting local inflammation. Mol Ther Methods Clin Dev 20:660-674 (2021). PubMed: 33718516
  • Mori T  et al. Effect of Myd88 deficiency on gene expression profiling in salivary glands of female non-obese diabetic (NOD) mice. J Oral Biosci 63:192-198 (2021). PubMed: 33933610
  • Boyd MM  et al. Pexidartinib treatment in Alexander disease model mice reduces macrophage numbers and increases glial fibrillary acidic protein levels, yet has minimal impact on other disease phenotypes. J Neuroinflammation 18:67 (2021). PubMed: 33685480
  • Li Z  et al. Uterine Scarring Leads to Adverse Pregnant Consequences by Impairing the Endometrium Response to Steroids. Endocrinology 161:N/A (2020). PubMed: 32976565
  • Wagner M  et al. Tumor-Derived Lactic Acid Contributes to the Paucity of Intratumoral ILC2s. Cell Rep 30:2743-2757.e5 (2020). PubMed: 32101749
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-10 of 14 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [CD3-12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution (S1699) - 20min @ ~90oC
Permeabilization
Yes - Wash buffer, blocking solution and secondary diluent was 1X TBS with 0.1% Tween-20
Specification
Spleen
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
10% neutral buffered formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 26 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [CD3-12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH6.0
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 30 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [CD3-12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain (MS tissue))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
Brain (MS tissue)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 12 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [CD3-12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Hannes Kaddatz

Verified customer

Submitted Dec 31 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [CD3-12]

Average
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 02 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [CD3-12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 05 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [CD3-12]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Trigeminal Ganglia)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0 + .05% Tween-20
Permeabilization
Yes - 0.5% Triton-X-100
Specification
Trigeminal Ganglia
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Kevin Egan

Verified customer

Submitted Mar 22 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [CD3-12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
10% NBF
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 02 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [CD3-12]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Thymus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH6.0
Permeabilization
No
Specification
Thymus
Blocking step
DakoCytomation X0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Hongwei Shao

Verified customer

Submitted Aug 28 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [CD3-12]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus monkey Tissue sections (Lymph Node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris base/EDTA
Permeabilization
Yes - Tween 20
Specification
Lymph Node
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 14 2017

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