Immunocytochemistry/ Immunofluorescence abreview for Anti-CD3 antibody [CD3-12]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Peripheral blood mononuclear cells (PBMCs))
Permeabilization
Yes - 0.5% TX100
Specification
Peripheral blood mononuclear cells (PBMCs)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% TX100, 1% goat serum, 1X PBS
Dilution
1/500

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: goat anti rat antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
T lymphocytes expressing CD3 epitope are labeled green. Nuclei were stained with DAPI.

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Nov 04 2016

Sign up