Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [CD3-12]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 50mM Borate Buffer with 1mM NaCl and 1mM EDTA - pH 8.0
Permeabilization
No
Specification
Spleen
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 23°C
Fixative
Formaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS with 2% serum and 0.1% tween-20
Dilution
1/200

Secondary antibody

Dilution
1/200

Additional data

Additional Notes
Secondary antibody was applied for 2 hours at room temperature. Nuclei were stained with DAPI.

Matthew Brown

Verified customer

Submitted Mar 13 2017

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