Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [CD3-12]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus monkey Tissue sections (Lymph Node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris base/EDTA
Permeabilization
Yes - Tween 20
Specification
Lymph Node
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: in humidified chamber
Dilution
1/500

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-rat IgG Af546
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 546

Additional data

Additional Notes
Some background staining, but overall a great stain!

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Submitted Apr 14 2017

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