Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [CD3-12]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Trigeminal Ganglia)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0 + .05% Tween-20
Permeabilization
Yes - 0.5% Triton-X-100
Specification
Trigeminal Ganglia
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 1% Normal goat serum
Dilution
1/100

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondar
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Additional data

Additional Notes
Tissue fixed in freshly made 4% formaldehyde. HIER in kitchen pressure cooker, maintained above 100 degrees C for 5 mins, cooled in buffer, and washed with PBS+.05% Tween-20.
PubMed ID
29175626: View

Dr. Kevin Egan

Verified customer

Submitted Mar 22 2018

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