Anti-CD3 antibody [F7.2.38] (ab17143)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [F7.2.38] (ab17143)](https://www.abcam.com/ps/products/17/ab17143/Images/ab17143-2-anti-cd3-antibody-f7-2-38-immunohistochemistry-tonsil-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [F7.2.38] (ab17143)")
Key features and details
- Mouse monoclonal [F7.2.38] to CD3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD3 antibody [F7.2.38]
See all CD3 primary antibodies -
Description
Mouse monoclonal [F7.2.38] to CD3 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) (Human).
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Positive control
- IHC-P: Human tonsil tissue.
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General notes
This antibody labels CD3 epsilon and is a useful tool for the identification of T cells and related neoplasms
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant, 1% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Primary antibody notes
This antibody labels CD3 epsilon and is a useful tool for the identification of T cells and related neoplasms -
Clonality
Monoclonal -
Clone number
F7.2.38 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
1/10 - 1/25. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
We suggest an incubation period of 30-60 minutes at room temperature. |
| Notes |
|---|
|
IHC-P
1/10 - 1/25. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. We suggest an incubation period of 30-60 minutes at room temperature. |
Target
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Function
The CD3 complex mediates signal transduction. -
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
Sequence similarities
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Omim: 186740 Human
- Omim: 186780 Human
- Omim: 186790 Human
- Omim: 186830 Human
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [F7.2.38] (ab17143)Paraffin embedded human tonsil tissue stained for CD3 using ab17143 in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [F7.2.38] (ab17143)](https://www.abcam.com/ps/products/17/ab17143/Images/ab17143-25959-anti-cd3-antibody-f7238-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [F7.2.38] (ab17143)ab17143 staining CD3 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab17143 has been referenced in 14 publications.
- Mitsui I et al. Chronic Cholecystitis of Dogs: Clinicopathologic Features and Relationship with Liver. Animals (Basel) 11:N/A (2021). IHC-P ; Dog . PubMed: 34828055
- Gudjonsson JE et al. Contribution of plasma cells and B cells to hidradenitis suppurativa pathogenesis. JCI Insight 5:N/A (2020). PubMed: 32853177
- Benner M et al. Clusters of Tolerogenic B Cells Feature in the Dynamic Immunological Landscape of the Pregnant Uterus. Cell Rep 32:108204 (2020). PubMed: 32997982
- Shao S et al. IFN-? enhances cell-mediated cytotoxicity against keratinocytes via JAK2/STAT1 in lichen planus. Sci Transl Med 11:N/A (2019). PubMed: 31554739
- Zhang L et al. Chimeric antigen receptor (CAR) T cells targeting a pathogenic MHC class II:peptide complex modulate the progression of autoimmune diabetes. J Autoimmun 96:50-58 (2019). PubMed: 30122420