Key features and details
- Mouse monoclonal [FN-18] to CD3
- Suitable for: IHC-Fr, Functional Studies, Flow Cyt
- Reacts with: Baboon, Cynomolgus monkey, Rhesus monkey
- Isotype: IgG1
Product nameAnti-CD3 antibody [FN-18]
See all CD3 primary antibodies
DescriptionMouse monoclonal [FN-18] to CD3
Tested applicationsSuitable for: IHC-Fr, Functional Studies, Flow Cytmore details
Species reactivityReacts with: Baboon, Cynomolgus monkey, Rhesus monkey
Does not react with: Mouse, Rat, Human
Rhesus Macaque peripheral blood limphocytes (PBL).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: PBS, 4.275% Trehalose
Concentration information loading...
Purification notesIon exchange chromatography
Our Abpromise guarantee covers the use of ab25109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Functional Studies||Use at an assay dependent concentration. Inhibition of ConA/PMA induced proliferation: 10µg/ml.Mitogenic sudies: 1 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionThe CD3 complex mediates signal transduction.
Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Sequence similaritiesContains 1 ITAM domain.
- Information by UniProt
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
Immunohistochemistry (Frozen sections) analysis of lymph node tissue from Rhesus macaque labeling CD3 with ab25109 at 1/100 dilution. Tissue was fixed in acetone, blocked using 10% goat serum/PBS for 15 min at 21°C, then incubated with Anti-CD3 antibody [FN-18] (ab25109) at 1/100 dilution for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-mouse polyclonal was used as the secondary antibody.
ab25109 has been referenced in 2 publications.
- Frencher JT et al. HMBPP-deficient Listeria mutant immunization alters pulmonary/systemic responses, effector functions, and memory polarization of V?2Vd2 T cells. J Leukoc Biol 96:957-67 (2014). PubMed: 25114162
- Stebbings R et al. "Cytokine storm" in the phase I trial of monoclonal antibody TGN1412: better understanding the causes to improve preclinical testing of immunotherapeutics. J Immunol 179:3325-31 (2007). Flow Cyt ; Cynomolgus monkey . PubMed: 17709549