Anti-CD3 antibody [KT3] (ab33429)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-CD3 antibody [KT3]
See all CD3 primary antibodies -
DescriptionRat monoclonal [KT3] to CD3
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Host speciesRat
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Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Frmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse
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Immunogen
Tissue/ cell preparation mature T cells (Mouse)
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General notes
ab33429 may be used to trigger proliferation and cytotoxicity of CD3 positive cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
PurityProtein G purified
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Purification notesPurified from tissue culture supernatant.
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Primary antibody notesab33429 may be used to trigger proliferation and cytotoxicity of CD3 positive cells.
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ClonalityMonoclonal
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Clone numberKT3
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MyelomaNS0
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IsotypeIgG2a
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab33429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | 1/100. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| IHC-Fr | Use at an assay dependent concentration. |
Application notes
Is unsuitable for IHC-P.
Target
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FunctionThe CD3 complex mediates signal transduction.
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Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
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Sequence similaritiesContains 1 ITAM domain.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 12500 Mouse
- Entrez Gene: 12501 Mouse
- Entrez Gene: 12502 Mouse
- Entrez Gene: 12503 Mouse
- SwissProt: P04235 Mouse
- SwissProt: P11942 Mouse
- SwissProt: P22646 Mouse
- SwissProt: P24161 Mouse
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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![Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [KT3] (ab33429)](https://www.abcam.com/ps/products/33/ab33429/Images/CD3-Primary-antibodies-ab33429-2.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [KT3] (ab33429)Image courtesy of an anonymous Abreviewab33429 staining CD3 in mouse spleen tissue by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/250 dilution for 12 hours at 4°C. A goat anti-rabbit IgG conjugated to Alexa Fluor®488 was used as secondary antibody at a 1/250 dilution.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Kumar L et al. Leucine-rich repeat containing 8A (LRRC8A) is essential for T lymphocyte development and function. J Exp Med 211:929-42 (2014). Read more (PubMed: 24752297) »
