Anti-CD3 antibody [KT3] (ab33429)
![Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [KT3] (ab33429)](https://www.abcam.com/ps/products/33/ab33429/Images/ab33429-46788-anti-cd3-antibody-kt3-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [KT3] (ab33429)")
Key features and details
- Rat monoclonal [KT3] to CD3
- Suitable for: IHC-Fr, ICC/IF, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Related conjugates and formulations
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD3 antibody [KT3]
See all CD3 primary antibodies -
Description
Rat monoclonal [KT3] to CD3 -
Host species
Rat -
Tested applications
Suitable for: IHC-Fr, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD3. mature T cells (Mouse)
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General notes
ab33429 may be used to trigger proliferation and cytotoxicity of CD3 positive cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Primary antibody notes
ab33429 may be used to trigger proliferation and cytotoxicity of CD3 positive cells. -
Clonality
Monoclonal -
Clone number
KT3 -
Myeloma
NS0 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33429 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| Flow Cyt |
Use at an assay dependent concentration.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
The CD3 complex mediates signal transduction. -
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
Sequence similarities
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12500 Mouse
- Entrez Gene: 12501 Mouse
- Entrez Gene: 12502 Mouse
- Entrez Gene: 12503 Mouse
- SwissProt: P04235 Mouse
- SwissProt: P11942 Mouse
- SwissProt: P22646 Mouse
- SwissProt: P24161 Mouse
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [KT3] (ab33429)Image courtesy of an anonymous Abreview
ab33429 staining CD3 in mouse spleen tissue by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/250 dilution for 12 hours at 4°C. A goat anti-rat IgG conjugated to Alexa Fluor®488 was used as secondary antibody at a 1/250 dilution.
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab33429 has been referenced in 5 publications.
- Gao M et al. Poldip2 mediates blood-brain barrier disruption and cerebral edema by inducing AQP4 polarity loss in mouse bacterial meningitis model. CNS Neurosci Ther 26:1288-1302 (2020). PubMed: 32790044
- Viitala M et al. Immunotherapeutic Blockade of Macrophage Clever-1 Reactivates the CD8+ T-cell Response against Immunosuppressive Tumors. Clin Cancer Res N/A:N/A (2019). PubMed: 30755440
- Chung WY et al. An ex vivo porcine spleen perfusion as a model of bacterial sepsis. ALTEX 36:29-38 (2019). PubMed: 30076761
- Yuzhalin AE et al. Proteomics analysis of the matrisome from MC38 experimental mouse liver metastases. Am J Physiol Gastrointest Liver Physiol 317:G625-G639 (2019). PubMed: 31545917
- Kumar L et al. Leucine-rich repeat containing 8A (LRRC8A) is essential for T lymphocyte development and function. J Exp Med 211:929-42 (2014). PubMed: 24752297
