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  1. Link

    cd3-antibody-kt3-ab33429.pdf

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Immunology Adaptive Immunity T Cells CD
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Anti-CD3 antibody [KT3] (ab33429)

  • Datasheet
Reviews (3) Submit a question References (5)

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Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [KT3] (ab33429)

    Key features and details

    • Rat monoclonal [KT3] to CD3
    • Suitable for: IHC-Fr, ICC/IF, Flow Cyt
    • Reacts with: Mouse
    • Isotype: IgG2a

    Conjugates logo Related conjugates and formulations

    PE

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-CD3 antibody [KT3]
      See all CD3 primary antibodies
    • Description

      Rat monoclonal [KT3] to CD3
    • Host species

      Rat
    • Tested applications

      Suitable for: IHC-Fr, ICC/IF, Flow Cytmore details
      Unsuitable for: IHC-P
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Tissue, cells or virus corresponding to Mouse CD3. mature T cells (Mouse)

    • General notes


      ab33429 may be used to trigger proliferation and cytotoxicity of CD3 positive cells.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      pH: 7.40
      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      Purified from tissue culture supernatant.
    • Primary antibody notes

      ab33429 may be used to trigger proliferation and cytotoxicity of CD3 positive cells.
    • Clonality

      Monoclonal
    • Clone number

      KT3
    • Myeloma

      NS0
    • Isotype

      IgG2a
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Stem Cells
      • Hematopoietic Progenitors
      • Hematopoietic Stem Cells
      • Human Lineage Negative
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells

    Associated products

    • Alternative Versions

      • PE Anti-CD3 antibody [KT3] (ab22268)
    • Compatible Secondaries

      • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
      • Goat Anti-Rat IgG H&L (HRP) (ab205720)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab33429 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr (1)
    Use at an assay dependent concentration.
    ICC/IF (1)
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.

    ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

    Notes
    IHC-Fr
    Use at an assay dependent concentration.
    ICC/IF
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.

    ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

    Application notes
    Is unsuitable for IHC-P.

    Target

    • Function

      The CD3 complex mediates signal transduction.
    • Involvement in disease

      Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    • Sequence similarities

      Contains 1 ITAM domain.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P04234 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12500 Mouse
      • Entrez Gene: 12501 Mouse
      • Entrez Gene: 12502 Mouse
      • Entrez Gene: 12503 Mouse
      • SwissProt: P04235 Mouse
      • SwissProt: P11942 Mouse
      • SwissProt: P22646 Mouse
      • SwissProt: P24161 Mouse
      • SwissProt: P29020 Mouse
      • Unigene: 4527 Mouse
      see all
    • Alternative names

      • 4930549J05Rik antibody
      • A430104F18Rik antibody
      • AW552088 antibody
      • Cd247 antibody
      • CD247 antigen antibody
      • CD247 antigen, zeta subunit antibody
      • CD247 molecule antibody
      • CD3 antibody
      • CD3 antigen, delta subunit antibody
      • CD3 delta antibody
      • CD3 epsilon antibody
      • CD3 eta antibody
      • CD3 gamma antibody
      • CD3 molecule delta polypeptide antibody
      • CD3 molecule, epsilon polypeptide antibody
      • CD3 molecule, gamma polypeptide antibody
      • CD3 zeta antibody
      • CD3-DELTA antibody
      • CD3d antibody
      • CD3D antigen delta polypeptide antibody
      • CD3d antigen, delta polypeptide (TiT3 complex) antibody
      • CD3d molecule delta antibody
      • CD3d molecule delta CD3 TCR complex antibody
      • CD3d molecule, delta (CD3-TCR complex) antibody
      • CD3D_HUMAN antibody
      • CD3E antibody
      • CD3e antigen antibody
      • CD3E antigen epsilon polypeptide antibody
      • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
      • CD3e molecule epsilon antibody
      • CD3e molecule epsilon CD3 TCR complex antibody
      • CD3e molecule, epsilon (CD3-TCR complex) antibody
      • CD3epsilon antibody
      • CD3G antibody
      • CD3g antigen antibody
      • CD3G antigen gamma polypeptide antibody
      • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
      • CD3g molecule gamma antibody
      • CD3g molecule gamma CD3 TCR complex antibody
      • CD3g molecule, gamma (CD3-TCR complex) antibody
      • CD3H antibody
      • CD3Q antibody
      • CD3Z antibody
      • CD3zeta antibody
      • Ctg3 antibody
      • FLJ17620 antibody
      • FLJ17664 antibody
      • FLJ18683 antibody
      • FLJ79544 antibody
      • FLJ94613 antibody
      • IMD19 antibody
      • Leu-4 antibody
      • MGC138597 antibody
      • OKT3, delta chain antibody
      • OTTHUMP00000032544 antibody
      • T cell receptor antibody
      • T cell receptor T3 delta chain antibody
      • T cell receptor T3 gamma chain antibody
      • T cell receptor T3 zeta chain antibody
      • T cell receptor zeta chain antibody
      • T cell surface antigen T3/Leu 4 epsilon chain antibody
      • T cell surface glycoprotein CD3 antibody
      • T cell surface glycoprotein CD3 delta chain antibody
      • T cell surface glycoprotein CD3 epsilon chain antibody
      • T cell surface glycoprotein CD3 gamma chain antibody
      • T cell surface glycoprotein CD3 zeta chain antibody
      • T-cell antigen receptor complex, delta subunit of T3 antibody
      • T-cell antigen receptor complex, epsilon subunit of T3 antibody
      • T-cell antigen receptor complex, gamma subunit of T3 antibody
      • T-cell antigen receptor complex, zeta subunit of CD3 antibody
      • T-cell receptor T3 delta chain antibody
      • T-cell receptor T3 gamma chain antibody
      • T-cell surface antigen T3/Leu-4 epsilon chain antibody
      • T-cell surface glycoprotein CD3 delta chain antibody
      • T-cell surface glycoprotein CD3 epsilon chain antibody
      • T-cell surface glycoprotein CD3 gamma chain antibody
      • T3 antibody
      • T3d antibody
      • T3e antibody
      • T3g antibody
      • T3z antibody
      • TCRE antibody
      • TCRk antibody
      • Tcrz antibody
      • TCRzeta antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [KT3] (ab33429)
      Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [KT3] (ab33429)Image courtesy of an anonymous Abreview

      ab33429 staining CD3 in mouse spleen tissue by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/250 dilution for 12 hours at 4°C. A goat anti-rat IgG conjugated to Alexa Fluor®488 was used as secondary antibody at a 1/250 dilution.

      See Abreview

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (5)

    Publishing research using ab33429? Please let us know so that we can cite the reference in this datasheet.

    ab33429 has been referenced in 5 publications.

    • Gao M  et al. Poldip2 mediates blood-brain barrier disruption and cerebral edema by inducing AQP4 polarity loss in mouse bacterial meningitis model. CNS Neurosci Ther 26:1288-1302 (2020). PubMed: 32790044
    • Viitala M  et al. Immunotherapeutic Blockade of Macrophage Clever-1 Reactivates the CD8+ T-cell Response against Immunosuppressive Tumors. Clin Cancer Res N/A:N/A (2019). PubMed: 30755440
    • Chung WY  et al. An ex vivo porcine spleen perfusion as a model of bacterial sepsis. ALTEX 36:29-38 (2019). PubMed: 30076761
    • Yuzhalin AE  et al. Proteomics analysis of the matrisome from MC38 experimental mouse liver metastases. Am J Physiol Gastrointest Liver Physiol 317:G625-G639 (2019). PubMed: 31545917
    • Kumar L  et al. Leucine-rich repeat containing 8A (LRRC8A) is essential for T lymphocyte development and function. J Exp Med 211:929-42 (2014). PubMed: 24752297

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD3 antibody [KT3]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (bone marrow)
    Specification
    bone marrow
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 19 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [KT3]

    Good
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (thymus)
    Specification
    thymus
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH 6.0
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 30 2010

    Immunohistochemistry (Frozen sections) abreview for Anti-CD3 antibody [KT3]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Spleen)
    Specification
    Spleen
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 30 2010

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