Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [KT3]

Good
Abreviews
This product is known to not work in this application or species.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (thymus)
Specification
thymus
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/250
Incubation time
9 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor® 488 goat anti-rat IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
DAPI was used for staining nucleus
Abcam response
Thank you for your valuable feedback. We are pleased to see this antibody has worked well in IHC-P in this experiment.

We do have previous feedback to indicate that this antibody provides inconsistent results in IHC-P and therefore we are not currently able to provide our guarantee for this application. We would appreciate and encourage any further feedback from customers who have used this antibody in IHC-P to enable us to collect more data. You can contact us at technical@abcam.com or send an Abreview.

Abcam user community

Verified customer

Submitted Dec 30 2010

Sign up