To fix the cell, it was incubated in methanol (-20°C) for 10 min. And washed by PBS. DAPI was used for staining nuclei. To know whether I could use this antibody as marker for T-cell in bone marrow for future studies. I had tried three times with different condition(blocking time and serum concentration). When I used the serum (horse, 5% in PBS) as a blocking buffer was best.
Abcam user community
Submitted Jan 19 2011
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