Immunocytochemistry/ Immunofluorescence abreview for Anti-CD3 antibody [KT3]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (bone marrow)
Specification
bone marrow
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/400
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor® 488 goat anti-rat IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
To fix the cell, it was incubated in methanol (-20°C) for 10 min. And washed by PBS. DAPI was used for staining nuclei.
To know whether I could use this antibody as marker for T-cell in bone marrow for future studies. I had tried three times with different condition(blocking time and serum concentration). When I used the serum (horse, 5% in PBS) as a blocking buffer was best.

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Submitted Jan 19 2011

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