Key features and details
- Mouse monoclonal [MEM-57] to CD3
- Suitable for: Functional Studies, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-CD3 antibody [MEM-57]
See all CD3 primary antibodies
DescriptionMouse monoclonal [MEM-57] to CD3
SpecificityThis antibody reacts with epsilon-gamma and epsilon-delta dimers of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor for antigen (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. It reacts more strongly with blood gamma-delta T lymphocytes than with alpha-beta T lymphocytes.
Tested applicationsSuitable for: Functional Studies, IP, Flow Cytmore details
Species reactivityReacts with: Human
Human thymocytes and T lymphocytes
Activates T cells in vitro. This antibody is a highly effective immunosuppressive agent. In this it is similar to OKT3.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesThis product has been purified from tissue culture supernatant.
Primary antibody notesActivates T cells in vitro. This antibody is a highly effective immunosuppressive agent. In this it is similar to OKT3.
Light chain typekappa
Our Abpromise guarantee covers the use of ab8090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration. Activates T cells in vitro; has a mitogenic effect on peripheral T lymphocytes.|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.01µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionThe CD3 complex mediates signal transduction.
Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Sequence similaritiesContains 1 ITAM domain.
- Information by UniProt
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
Human peripheral blood lymphocytes stained with ab8090 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.
Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD3 (MEM-57) Dy161. Gated on singlets.
ab8090 has been referenced in 7 publications.
- Garrett SM et al. Induction of a Th17 Phenotype in Human Skin-A Mimic of Dermal Inflammatory Diseases. Methods Protoc 2:N/A (2019). PubMed: 31164624
- Savitski MM et al. Multiplexed Proteome Dynamics Profiling Reveals Mechanisms Controlling Protein Homeostasis. Cell 173:260-274.e25 (2018). PubMed: 29551266
- Lipp AM et al. Lck mediates signal transmission from CD59 to the TCR/CD3 pathway in Jurkat T cells. PLoS One 9:e85934 (2014). Human . PubMed: 24454946
- Giese S & Marsh M Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells. PLoS Pathog 10:e1004189 (2014). Flow Cyt . PubMed: 24991932
- Bergamini G et al. A selective inhibitor reveals PI3K? dependence of T(H)17 cell differentiation. Nat Chem Biol 8:576-82 (2012). PubMed: 22544264
- Hilgert I et al. Therapeutic in vivo use of the A1-CD3 monoclonal antibody. Transplantation 55:435-8 (1993). PubMed: 8434399
- HorejsÃ V et al. Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74). Folia Biol (Praha) 34:23-34 (1988). PubMed: 2968928