Anti-CD3 antibody [OKT3] (ab86883)
Key features and details
- Mouse monoclonal [OKT3] to CD3
- Suitable for: Flow Cyt, Functional Studies
- Reacts with: Human
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-CD3 antibody [OKT3]
See all CD3 primary antibodies -
Description
Mouse monoclonal [OKT3] to CD3 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, Functional Studiesmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human lymphocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
OKT3 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (1) |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| Functional Studies |
Use at an assay dependent concentration.
(Activation) |
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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Functional Studies
Use at an assay dependent concentration. (Activation) |
Target
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Function
The CD3 complex mediates signal transduction. -
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
Sequence similarities
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Omim: 186740 Human
- Omim: 186780 Human
- Omim: 186790 Human
- Omim: 186830 Human
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab86883 has been referenced in 8 publications.
- Chen Y et al. High Mobility Group Box 1 Contributes to the Acute Rejection of Liver Allografts by Activating Dendritic Cells. Front Immunol 12:679398 (2021). PubMed: 34177922
- Li YQ et al. Regulation of endothelial cell survival and death by the MAP kinase/ERK kinase kinase 3 - glyceraldehyde-3-phosphate dehydrogenase signaling axis. Cell Signal 58:20-33 (2019). PubMed: 30831195
- Ilinskaya AN et al. Nanoparticle physicochemical properties determine the activation of intracellular complement. Nanomedicine 17:266-275 (2019). PubMed: 30794962
- Gunawardene P et al. Association Between Circulating Osteogenic Progenitor Cells and Disability and Frailty in Older Persons: The Nepean Osteoporosis and Frailty Study. J Gerontol A Biol Sci Med Sci 71:1124-30 (2016). PubMed: 26525092
- Fischer N et al. Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG. Nat Commun 6:6113 (2015). PubMed: 25672245
- Ritchie MF et al. STIM1 is required for attenuation of PMCA-mediated Ca2+ clearance during T-cell activation. EMBO J 31:1123-33 (2012). PubMed: 22246182
- Egan KP et al. Role for circulating osteogenic precursor cells in aortic valvular disease. Arterioscler Thromb Vasc Biol 31:2965-71 (2011). Flow Cyt ; Human . PubMed: 21903944
- Esteitie R et al. Effect of fluticasone furoate on interleukin 6 secretion from adenoid tissues in children with obstructive sleep apnea. Arch Otolaryngol Head Neck Surg 137:576-82 (2011). PubMed: 21690510