Application
Western blot
Sample
Human Cell lysate - whole cell (Human peripheral blood mononuclear cells)
Loading amount
25 µg
Specification
Human peripheral blood mononuclear cells
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS-Tween (0.5%)
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse IgG (HRP)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
ECL+
Exposure
8 minute(s) and 0 second(s)
Bands
Specific: 23 kDa
Positive control
Human peripheral blood mononuclear cell extract
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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Submitted Oct 30 2012