Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human peripheral blood mononuclear cells)
Specification
Human peripheral blood mononuclear cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488 Goat anti-mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/250
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Abcam user community
Verified customer
Submitted Jan 25 2013