Recombinant Anti-CD3 antibody [SP162] (ab135372)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-CD3 antibody [SP162]
See all CD3 primary antibodies -
DescriptionRabbit monoclonal [SP162] to CD3
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig
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Immunogen
Synthetic peptide within Human CD3 aa 150 to the C-terminus (internal sequence). The exact sequence is proprietary.
Database link: P07766 -
Positive control
- IHC-P: Human tonsil tissue. Flow Cyt: Jurkat and Mouse Splenocyte cells WB: Jurkat whole cell lysate (ab7899). ICC/IF: Jurkat and EL4.IL-2 cells
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General notes
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
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Storage bufferpH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
PurityProtein A/G purified
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Purification notesPurified from TCS by protein A/G.
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ClonalityMonoclonal
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Clone numberSP162
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab135372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/10. | |
| IHC-P | 1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 10 minutes at room temperature. | |
| Flow Cyt | 1/150 - 1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | 1/150. Predicted molecular weight: 19 kDa. Incubate for 1 hour at room temperature. |
Target
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FunctionThe CD3 complex mediates signal transduction.
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Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
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Sequence similaritiesContains 1 ITAM domain.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Entrez Gene: 12500 Mouse
- Entrez Gene: 12501 Mouse
- Entrez Gene: 12502 Mouse
- Entrez Gene: 12503 Mouse
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Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (ab135372)](https://www.abcam.com/ps/products/135/ab135372/Images/ab135372-4-antiCD3antibodySP162-immunocytochemistry-Jurkat-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (ab135372)Immunocytochemistry/ Immunofluorescence analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling CD3 with purified ab135372 at 1/10 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)](https://www.abcam.com/ps/products/135/ab135372/Images/ab135372-6-antiCD3antibodySP162-flowcytometry-Jurkat-human.jpg)
Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD3 with purified ab135372 at 1/200 dilution (0.60 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (ab135372)](https://www.abcam.com/ps/products/135/ab135372/Images/CD3-Primary-antibodies-ab135372-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (ab135372)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD3 with ab135372 at a 1/150 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (ab135372)](https://www.abcam.com/ps/products/135/ab135372/Images/ab135372-5-antiCD3antibodySP162-immunocytochemistry-EL41L2-mouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (ab135372)Immunocytochemistry/ Immunofluorescence analysis of EL4.IL-2 (mouse lymphoma T lymphocyte) cells labeling CD3 with purified ab135372 at 1/10 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)](https://www.abcam.com/ps/products/135/ab135372/Images/ab135372-7-antiCD3antibodySP162-flowcytometry-splenocyte-mouse.jpg)
Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)Flow Cytometry analysis of Mouse Splenocyte cells labeling CD3 with purified ab135372 at 1/20 dilution (5.95 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
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![Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)](https://www.abcam.com/ps/products/135/ab135372/Images/ab135372-215442-ab135372-Flow.jpg)
Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)Flow cytometric analysis of rabbit anti-CD3 (SP162) antibody ab135372 (1/150) in Jurkat (Human T cell leukemia cell line from peripheral blood) cells (green) compared to negative control of rabbit IgG (blue).
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![Western blot - Anti-CD3 antibody [SP162] (ab135372)](https://www.abcam.com/ps/products/135/ab135372/Images/CD3-Primary-antibodies-ab135372-3.jpg)
Western blot - Anti-CD3 antibody [SP162] (ab135372)Anti-CD3 antibody [SP162] (ab135372) at 1/150 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Predicted band size: 19 kDa
References
This product has been referenced in:
- Fisher TS et al. A CD3-bispecific molecule targeting P-cadherin demonstrates T cell-mediated regression of established solid tumors in mice. Cancer Immunol Immunother 67:247-259 (2018). Read more (PubMed: 29067496) »
