Recombinant Anti-CD3 antibody [SP162] (ab135372)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (ab135372)](https://www.abcam.com/ps/products/135/ab135372/Images/ab135372-149583-anti-cd3-antibody-sp162-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (ab135372)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP162] to CD3
- Suitable for: ICC/IF, IHC-P, Flow Cyt, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD3 antibody [SP162]
See all CD3 primary antibodies -
Description
Rabbit monoclonal [SP162] to CD3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig
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Immunogen
Synthetic peptide within Human CD3 aa 150 to the C-terminus (internal sequence). The exact sequence is proprietary.
Database link: P07766 -
Positive control
- IHC-P: Human tonsil tissue. Flow Cyt: Jurkat and Mouse Splenocyte cells WB: Jurkat whole cell lysate (ab7899). ICC/IF: Jurkat and EL4.IL-2 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP162 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab135372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/10.
|
|
| IHC-P | (4) |
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 10 minutes at room temperature.
|
| Flow Cyt |
1/150 - 1/200.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/150. Predicted molecular weight: 19 kDa. Incubate for 1 hour at room temperature.
|
| Notes |
|---|
|
ICC/IF
1/10. |
|
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 10 minutes at room temperature. |
|
Flow Cyt
1/150 - 1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/150. Predicted molecular weight: 19 kDa. Incubate for 1 hour at room temperature. |
Target
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Function
The CD3 complex mediates signal transduction. -
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
Sequence similarities
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Entrez Gene: 12500 Mouse
- Entrez Gene: 12501 Mouse
- Entrez Gene: 12502 Mouse
- Entrez Gene: 12503 Mouse
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (ab135372)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD3 with ab135372 at a 1/150 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (ab135372)](https://www.abcam.com/ps/products/135/ab135372/Images/ab135372-4-antiCD3antibodySP162-immunocytochemistry-Jurkat-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (ab135372)Immunocytochemistry/ Immunofluorescence analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling CD3 with purified ab135372 at 1/10 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)](https://www.abcam.com/ps/products/135/ab135372/Images/ab135372-6-antiCD3antibodySP162-flowcytometry-Jurkat-human.jpg)
Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD3 with purified ab135372 at 1/200 dilution (0.60 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (ab135372)](https://www.abcam.com/ps/products/135/ab135372/Images/ab135372-5-antiCD3antibodySP162-immunocytochemistry-EL41L2-mouse.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (ab135372)Immunocytochemistry/ Immunofluorescence analysis of EL4.IL-2 (mouse lymphoma T lymphocyte) cells labeling CD3 with purified ab135372 at 1/10 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)](https://www.abcam.com/ps/products/135/ab135372/Images/ab135372-7-antiCD3antibodySP162-flowcytometry-splenocyte-mouse.jpg)
Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)Flow Cytometry analysis of Mouse Splenocyte cells labeling CD3 with purified ab135372 at 1/20 dilution (5.95 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
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![Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)](https://www.abcam.com/ps/products/135/ab135372/Images/ab135372-215442-anti-cd3-antibody-sp162-flow-cytometry.jpg)
Flow Cytometry - Anti-CD3 antibody [SP162] (ab135372)Flow cytometric analysis of rabbit anti-CD3 (SP162) antibody ab135372 (1/150) in Jurkat (Human T cell leukemia cell line from peripheral blood) cells (green) compared to negative control of rabbit IgG (blue).
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![Western blot - Anti-CD3 antibody [SP162] (ab135372)](https://www.abcam.com/ps/products/135/ab135372/Images/ab135372-149584-anti-cd3-antibody-sp162-western-blot.jpg)
Western blot - Anti-CD3 antibody [SP162] (ab135372)Anti-CD3 antibody [SP162] (ab135372) at 1/150 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Predicted band size: 19 kDa -
![Anti-CD3 antibody [SP162] (ab135372)](https://www.abcam.com/ps/products/135/ab135372/Images/ab135372-8-benefits-of-recombinant-antibodies.png)
Anti-CD3 antibody [SP162] (ab135372)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab135372 has been referenced in 21 publications.
- Zhang H et al. Inhibition of galectin-3 augments the antitumor efficacy of PD-L1 blockade in non-small-cell lung cancer. FEBS Open Bio 11:911-920 (2021). PubMed: 33455075
- Liu Z et al. Elimination of macrophages reduces glutaraldehyde-fixed porcine heart valve degeneration in mice subdermal model. Pharmacol Res Perspect 9:e00716 (2021). PubMed: 33523576
- Liu Y et al. Tumour heterogeneity and intercellular networks of nasopharyngeal carcinoma at single cell resolution. Nat Commun 12:741 (2021). PubMed: 33531485
- Gong W et al. CCL4-mediated targeting of spleen tyrosine kinase (Syk) inhibitor using nanoparticles alleviates inflammatory bowel disease. Clin Transl Med 11:e339 (2021). PubMed: 33634985
- Ma YS et al. M2 macrophage-derived exosomal microRNA-155-5p promotes the immune escape of colon cancer by downregulating ZC3H12B. Mol Ther Oncolytics 20:484-498 (2021). PubMed: 33718596