Overview

  • Product name
    Anti-CD3 antibody [SP7]
    See all CD3 primary antibodies
  • Description
    Rabbit monoclonal [SP7] to CD3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IHC-P, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Sheep, Rabbit, Horse, Chicken, Cow, Cat, Dog, Baboon, Woodchuck
  • Immunogen

    Synthetic peptide corresponding to CD3.

  • Epitope
    aa 156-168 of epsilon chain of Human CD3 protein (intracytoplasmic).
  • Positive control
    • WB: Recombinant Human CD3 epsilon protein (ab114153), Jurkat whole cell lysate. Human, mouse and rat thymus tissue lysate. IHC-P: Pig and rat spleen tissue. Human tonsil tissue. Mouse epidydimal fat pad tissue. Rat infarcted heart tissue. Flow Cytometry: Human peripheral blood lymphocytes. Jurkat cells. IHC-Fr: Mouse brain tissue.
  • General notes

    We recommend ab135372 as an alternative.

    This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab16669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

We recommend using Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody.

IHC-Fr Use at an assay dependent concentration. PubMed: 18658050
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

WB 1/200. Predicted molecular weight: 23 kDa.
IHC-FoFr Use at an assay dependent concentration.

Target

  • Function
    The CD3 complex mediates signal transduction.
  • Involvement in disease
    Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • Sequence similarities
    Contains 1 ITAM domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • Cd247 antibody
    • CD247 antigen antibody
    • CD247 antigen, zeta subunit antibody
    • CD247 molecule antibody
    • CD3 antibody
    • CD3 antigen, delta subunit antibody
    • CD3 delta antibody
    • CD3 epsilon antibody
    • CD3 eta antibody
    • CD3 gamma antibody
    • CD3 molecule delta polypeptide antibody
    • CD3 molecule, epsilon polypeptide antibody
    • CD3 molecule, gamma polypeptide antibody
    • CD3 zeta antibody
    • CD3-DELTA antibody
    • CD3d antibody
    • CD3D antigen delta polypeptide antibody
    • CD3d antigen, delta polypeptide (TiT3 complex) antibody
    • CD3d molecule delta antibody
    • CD3d molecule delta CD3 TCR complex antibody
    • CD3d molecule, delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • CD3E antibody
    • CD3e antigen antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule epsilon CD3 TCR complex antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3epsilon antibody
    • CD3G antibody
    • CD3g antigen antibody
    • CD3G antigen gamma polypeptide antibody
    • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
    • CD3g molecule gamma antibody
    • CD3g molecule gamma CD3 TCR complex antibody
    • CD3g molecule, gamma (CD3-TCR complex) antibody
    • CD3H antibody
    • CD3Q antibody
    • CD3Z antibody
    • CD3zeta antibody
    • Ctg3 antibody
    • FLJ17620 antibody
    • FLJ17664 antibody
    • FLJ18683 antibody
    • FLJ79544 antibody
    • FLJ94613 antibody
    • IMD19 antibody
    • Leu-4 antibody
    • MGC138597 antibody
    • OKT3, delta chain antibody
    • OTTHUMP00000032544 antibody
    • T cell receptor antibody
    • T cell receptor T3 delta chain antibody
    • T cell receptor T3 gamma chain antibody
    • T cell receptor T3 zeta chain antibody
    • T cell receptor zeta chain antibody
    • T cell surface glycoprotein CD3 antibody
    • T cell surface glycoprotein CD3 delta chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T cell surface glycoprotein CD3 gamma chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, delta subunit of T3 antibody
    • T-cell antigen receptor complex, epsilon subunit of T3 antibody
    • T-cell antigen receptor complex, gamma subunit of T3 antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell receptor T3 gamma chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T-cell surface glycoprotein CD3 gamma chain antibody
    • T3 antibody
    • T3d antibody
    • T3e antibody
    • T3g antibody
    • T3z antibody
    • TCRE antibody
    • TCRk antibody
    • Tcrz antibody
    • TCRzeta antibody
    see all

Images

  • Immunohistochemical analysis of Formaldehyde fixed, paraffin-embedded pig spleen tissue sections labelling CD3 with ab16669 at a dilution of 1/100. Biotin conjugated Goat Anti-Rabbit IgG was used as the secondary antibody. Antigen retrieval was heat mediated using citric acid.

    See Abreview

  • Human peripheral blood lymphocytes stained with ab16669 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab16669, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

  • All lanes : Anti-CD3 antibody [SP7] (ab16669) at 1/25 dilution

    Lane 1 : THP1 whole cell lysate (-ve control)
    Lane 2 : Raji whole cell lysate (-ve control)
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : Human Thymus tissue lysate
    Lane 5 : Mouse Thymus tissue lysate
    Lane 6 : Rat Thymus tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Lanes 1 - 6: Merged signal (red and green). Green – ab16669 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.

     

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab16669 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.

  • IHC using multi-spectral imaging on human lymph node (A-C) obtained from men with mCRPC. A) H+E staining and B) single-color images (plus nuclear stain; DAPI) of CD3 (ab16669), CD8 (ab101500), CD163, PD-L1, cytokeratin (CK), DAPI and C) merged.  H+E stating at 20X magnification; multi-spectral images 200X magnification.

  • Immunohistochemical analysis of Formaldehyde fixed, frozen rat spleen tissue sections labelling CD3 with ab16669 at a dilution of 1/500. Biotin conjugated Goat Anti-Rabbit IgG at 1/300 dilution was used as the secondary antibody. 

    Cryostat sections (10 microns thick) of fresh frozen spleen were dried overnight. They were fixed in 10% Formalin in PBS pH7 for 15 minutes.
    After secondary antibody incubation ( Goat anti-rabbit biotin) , streptavidin-Alexa 594 was applied.

    See Abreview

  • Immunohistochemical (Formalin / PFA fixed paraffin-embedded sections) staining of of Tonsil tissue labeling CD3 (SP7) with ab16669 at 1/150 dilution.

  • Immunohistochemical analysis of Formaldehyde fixed, paraffin-embedded rat spleen tissue sections labelling CD3 with ab16669 at a dilution of 1/100. Biotin conjugated Goat Anti-Rabbit IgG at 1/300 dilution was used as the secondary antibody. Antigen retrieval was heat mediated using citric acid.

    See Abreview

  • Ab16669 staining CD3 in Mouse Epidydimal fat pad tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue sections were fixed with formaldehyde, blocked with 5% serum for 4 hours at 25°C and permeabilized with Triton X-100. Samples were incubated with primary antibody (1/100 in PBST with BSA and goat serum) for 4°C at 12 hours. An Alexa Fluor® 568 goat anti-rabbit IgG (H + L) cross adsorbed was used as the secondary antibody.

    See Abreview

  • Flow cytometric analysis of rabbit anti-CD3 (SP7) antibody ab16669 (1/100) in Jurkats cells (green) compare to negative control of rabbit IgG (blue).

  • Human tonsil stained with ab16669.
  • ab16669 staining rat infarcted heart tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Myocardial infarction was produced in a rat model following the ligation of the left anterior descending (LAD) coronary artery. Tissue was harvested 6 w following infarct, fixed with Histochoice for 72 hr, paraffin sectioned and the slide was then baked prior to CD3 staining. ab16669 at 1/200 was incubated overnight at 4°C. The image was taken with a confocal laser scanning microscope and shows cells giving strong inmmunofluorescence staining for CD3 antigen (green), indicating presence of cells of T-lymphocytes origin in the infarct zone of the heart tissue, counterstained nuclei with DAPI (blue). Note, CD3 tended to be present in nests of 2-5 cells that were non-uniformly distributed in the infarct zone. In addition, the image shows that the CD3 localization is predominantly membrane based and to a certain extent intracytoplasmic.

    See Abreview

  • Anti-CD3 antibody [SP7] (ab16669) at 1/25 dilution + Jurkat cell lysate

    Predicted band size: 23 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?

  • ab16669 staining CD3 in murine brain tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in acetone, blocked using 5% serum for 30 minutes at 25°C and then incubated with ab16669 at a 1/200 dilution for 18 hours at 4°C. The secondary used was an undiluted HRP conjugated goat anti-rabbit polyclonal.

    See Abreview

  • Immunohistochemical analysis of Human tonsil tissue, staining CD3 (green) with ab16669.

    Antigen retrieval was performed by heat mediation in citrate buffer (pH 6) and blocked with 5% goat serum and 5% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) overnight at 4°C. A Cy3®-conjugated anti-rabbit IgG was used as the secondary antibody.

References

This product has been referenced in:
  • Luo N  et al. DNA methyltransferase inhibition upregulates MHC-I to potentiate cytotoxic T lymphocyte responses in breast cancer. Nat Commun 9:248 (2018). Read more (PubMed: 29339738) »
  • Höing B  et al. Stromal versus tumoral inflammation differentially contribute to metastasis and poor survival in laryngeal squamous cell carcinoma. Oncotarget 9:8415-8426 (2018). IHC-P . Read more (PubMed: 29492204) »
See all 149 Publications for this product

Customer reviews and Q&As

1-9 of 9 Q&A

Answer

can confirm that ab16669 CD3 antibody [SP7] is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.


I can confirm that for tissue culture supernatant, concentration of antibody is known to vary between 1 - 3 mg/ml


I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you.



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Answer

Thank you for contacting us.

I am sorry to confirm that because we carry over 90,000 products, it is not feasible for us to stock small sample sizes of our products to send on request.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our AbTrial program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Or you can find more information about this program at the following link:

www.abcam.com/abtrial

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:
https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Answer

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.

Could you please provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

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Answer

Thank you for your enquiry.

I can confirm that the Anti-CD3 antibody [SP7] (ab16669) antibody isprovided as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not always have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for tissue culture supernatant of rabbit monoclonals tends to be of a concentration ofaround 0.1 - 0.3 mg/ml. This may seem quite low however, the IgG produced by rabbits have greater affinity and greater sensitivity than mouse IgG (about 100-1000 fold) and at the same time the total amount of IgG produced by the rabbit hybridomas is generally lower than in mouse hybridomas.

Many 'veteran' antibody users are generally familiar with the IgG amount provided from mouse antibodies and can judge how many reactions they may get or need to use based on concentration. Unfortunately, this experience is not transferable to rabbit monoclonal antibodies because the concentration scales are generally very different.

Since the specificity and sensitivity is much higher with rabbit monoclonal antibodies, less IgG is required per reaction.

I would like to reassure you that while the amount of IgG may not be comparable to mouse monoclonal antibodies, customers are receiving a comparable number of reactions/tests/blots/stainings per tube.

There is some guidance on the datasheet of this product which offers starting point dilutions for different applications. Please note that these may need to be optimised for your particular experiment.

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for contacting us.

We currently do not offer products which meet each of the requirements that you have set. I assembled for you well characterized products which meet most of these requirements but each has some deficiency. Pricing is to be found near the top of the webpage for each product directly below the bold type face product name. There is not currently any special pricing at JHMI. Each datasheet contains all images which we currently have for the products. Any publications which we are aware of the product being used in are located behind the specific references tab or at the bottom of the page.

I would recommend rabbit antibodies as it appears you want to test in mouse however I have added mouse antibodies should you wish to use a mouse on mouse IHC kit such as theMouse on Mouse (M.O.M) Polymer IHC Kit ab127055:https://www.abcam.com/Mouse-on-Mouse-MOM-Polymer-IHC-Kit-ab127055.html



Unconjugated anti-CD3 that targets rat, mouse and pig produced in any animal:

ab16669; rabbit monoclonal; predicted based on sequence homology but not yet tested in Pig

https://www.abcam.com/CD3-antibody-SP7-ab16669.html

Or

ab8671; Mouse monoclonal; predicted but not tested in Pig based on sequence homology; not yet tested in IHC-P

https://www.abcam.com/CD3-antibody-B355-1-RIV-9-ab8671.html



Unconjugated anti-CD4 that targets rat, mouse and pig produced in that same animal:

Ab61131; Rabbit polyclonal; predicted but not tested in Pig, Mouse, Rat based on sequence homology

https://www.abcam.com/CD4-antibody-ab61131.html

Or

ab125711; Mouse monoclonal;predicted in pig; not yet tested in mouse

https://www.abcam.com/CD4-antibody-10B5-ab125711.html



Unconjugated anti-CD8 that targets rat, mouse and pig produced in that same animal:

Ab4055; Rabbit polyclonal, predicted but not tested in Pig, Mouse, Rat based on sequence homology

https://www.abcam.com/CD8-antibody-ab4055.html

Or

Ab17147; Mouse monoclonal, not yet tested in Mouse, Rat or Pig. I do not that the exact immunogen however the C-terminal end is well conserved in each species as such we predict this product may work in these species

https://www.abcam.com/CD8-antibody-144B-ab17147.html



Unconjugated anti-FOXP3 that targets rat, mouse and pig produced in that same animal:

ab75763; Rabbit polyclonal; not yet tested in rat and pig in IHC-P This product is the only rabbit host which is predicted to work in all these species;

https://www.abcam.com/FOXP3-antibody-ab75763.html

Or

ab22510; Mouse monoclonal; predicted but not yet tested in mouse and pig based on sequence homology

https://www.abcam.com/FOXP3-antibody-mAbcam-22510-ab22510.html



Horseradish peroxidase-conjugated anti-that same animal IgG:

Ab97051; Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-HRP-ab97051.html

Or

Ab97023; Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP)

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-HL-HRP-ab97023.html



For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you very much for your phone call.

The best antibodies we can recommend are as follows. Please note that the recommendations are based on immunogen sequence only we currently do not hold any rabbit specific data.

Anti CD3 : ab16669; I can provide Abreview image discount code and will also provide Abpromise guarantee on use for this ab.

Anti FOXP3: ab20034, ab22510 are best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

Anti CD4: ab61131, ab101530 and ab108329, are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

anti CD8: ab108343, ab123630 and ab86733, are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

Anti CD1A : ab76531, ab108309 and ab123133 are the best antibodies we can offer. Eligible for new species Abreview discount code however not eligible for refund in case it does not work.

Please also note that most of these antibodies are raised in Rabbit so there will be high back ground staining. Our recommendations to reduce the back ground staining are

- Use preadsorbed anti body e.g. ab7132, ab7083, ab

- Use (Fab)2 fragments antibodies e.g. ab6112, ab102296

- Incubate sections with affinity pure Fab fragment anti rabbit antibody before applying primary antibody

Please check the full details by clicking the links;

https://www.abcam.com/index.html?pageconfig=resource&rid=12722
https://www.abcam.com/index.html?pageconfig=resource&rid=12825
https://www.abcam.com/index.html?pageconfig=resource&rid=11465

The abreview discount code details can be found by clicking following link;

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount

Could you please select the antibodies you are interested in, I will then be happy in creating a discount code for each one of these.

I will look forward to hearing from you soon.

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Answer

Thank you for your email.

We have 4 rabbit antibodies that work in rat in IHC-P. I have listed them below:

ab16044 Anti-CD3 antibody
https://www.abcam.com/ab16044

ab16669 Anti-CD3 antibody [SP7]
https://www.abcam.com/ab16669

ab13628 Anti-CD3 antibody
https://www.abcam.com/ab13628

In order to help investigate the problems with ab5690 and see if there are any tips I can offer to help, would it be possible to find out a bit more information about your protocol? I have included some questions below. Looking forward to your reply.

Description of the problem (no staining, non-specific or incorrect staining, high background, etc)
Product lot number
Sample preparation (tissue/cell linetested , species, how these have been fixed, any antigen retrieval steps performed, permeabilization steps)
Blocking conditions (buffer used, blocking time/temperature)
Primary antibody conditions (manufacturer/species/target, diluent, dilution used, incubation time/temperature)
Secondary antibody conditions (manufacturer/species/target, diluent, dilution used, incubation time/temperature)
Wash steps (buffer used, time and number of washes)
Detection (manufacturer, detection method used: DAB/fluorescent /etc, any amplification step used)
Controls (positive/negative, no primary)
Any troubleshooting steps you have attempted, how many times you have attempted to run the experiment
Any order information that will help us locate your original order in our system (Abcam order number, PO number, date)

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Answer

Thank you for your enquiry. According to the on-line datasheet, this antibody is recommended to be stored at -20°C and it should be viable for up to 5 years. However, it is important to emphasize that our Abpromise guarantees that the product will work in the tested species and application for 6 months after the purchase when stored properly. I hope this helps and if I can assist further, please do not hesitate to contact me.

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Answer

Thank you for contacting us. The relative specificity of each antibody is difficult to assess without a side-by-side comparison. The data for the SP7 clone and the positive reviews we have received from customers are all encouraging. Staining of canine samples has not been assessed for ab16669. We expect reactivity based on homology of the immunogen with canine CD3 (77% identity) but this is not covered by our guarantee. The concentration of the SP7 tissue culture supernatant is not determined. We estimate specific antibody concentration for tissue culture supernatants in general to be about 50 ug/ml. Each lot is QC'd to ensure that there will be consistency in results from one lot to the next. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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