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Inquiry: Please can you give me the starting concentration of this antibody as we are needing to carry out a control test for non-specific binding. We have been using it at a dilution of 1/100 on our slides for biotin staining.
Asked on Jan 13 2017
can confirm that ab16669 CD3 antibody [SP7] is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.
I can confirm that for tissue culture supernatant, concentration of antibody is known to vary between 1 - 3 mg/ml
I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you.
Answered on Jan 13 2017