Immunohistochemistry (Frozen sections) abreview for Anti-CD3 antibody [SP7]

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Spleen)
Permeabilization
No
Specification
Spleen
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Dilution
1/500

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Cryostat sections ( 10 microns thick) of fresh frozen spleen were dried overnight. They were fixed in 10% Formalin in PBS pH7 for 15 minutes. After secondary antibody incubation ( Gt anti Rb -biotin) , stAvidin-Alexa 594 was applied.

Mr. Carl Hobbs

Verified customer

Submitted Dec 21 2016

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