Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [SP7]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
Permeabilization
Yes - Tween-20
Specification
Lymph node
Blocking step
RTU Power Vision blocking from Leica as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde

Other product details

Incubation time
45 minute(s) · Diluent: RTU Power Vision blocking from Leica
Dilution
1/150

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Invitrogen
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568

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Submitted Jun 25 2018

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