Key features and details
- Rabbit monoclonal [SP7] to CD3, prediluted
- Suitable for: WB, Flow Cyt, IHC-FoFr, IHC-Fr, IHC-P
- Reacts with: Mouse, Human, Cynomolgus monkey
- Isotype: IgG
Product nameAnti-CD3 antibody [SP7], prediluted
See all CD3 primary antibodies
DescriptionRabbit monoclonal [SP7] to CD3, prediluted
Specificityab21703 recognises CD3 epsilon chain. This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues.
Tested applicationsSuitable for: WB, Flow Cyt, IHC-FoFr, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Mouse, Human, Cynomolgus monkey
Predicted to work with: Rat, Sheep, Rabbit, Cow, Dog, Pig, Macaque monkey, Woodchuck
Synthetic peptide within Human CD3 aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: P07766
- Recombinant Human CD3 epsilon protein (ab114153) can be used as a positive control in WB. Tonsil tissue.
FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
This antibody is suitable for staining normal and neoplastic T cells.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tissue culture supernatant, Tris buffered saline, 1% BSA
Concentration information loading...
PurityTissue culture supernatant
Primary antibody notesThis antibody is suitable for staining normal and neoplastic T cells.
Our Abpromise guarantee covers the use of ab21703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/25. Predicted molecular weight: 19 kDa.|
|Flow Cyt||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
|IHC-P||1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use neat. Perform high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0.
FunctionThe CD3 complex mediates signal transduction.
Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Sequence similaritiesContains 1 ITAM domain.
- Information by UniProt
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining CD3 with ab21703.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thymus tissue, staining CD3 with ab21703.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Hodgkin's lymphoma tissue, staining CD3 with ab21703.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue, staining CD3 with ab21703.
ab21703 has been referenced in 16 publications.
- Schropp V et al. Contribution of LTi and TH17 cells to B cell aggregate formation in the central nervous system in a mouse model of multiple sclerosis. J Neuroinflammation 16:111 (2019). PubMed: 31138214
- Kisu I et al. Long-Term Outcome and Rejection After Allogeneic Uterus Transplantation in Cynomolgus Macaques. J Clin Med 8:N/A (2019). PubMed: 31581534
- Buss LA & Dachs GU Voluntary exercise slows breast tumor establishment and reduces tumor hypoxia in ApoE-/- mice. J Appl Physiol (1985) 124:938-949 (2018). PubMed: 29357514
- Wang L et al. An Acquired Vulnerability of Drug-Resistant Melanoma with Therapeutic Potential. Cell 173:1413-1425.e14 (2018). PubMed: 29754815
- Pillon A et al. Actinic keratosis modelling in mice: A translational study. PLoS One 12:e0179991 (2017). PubMed: 28662116