Key features and details
- Mouse monoclonal [UCH-T1] to CD3 - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CD3 antibody [UCH-T1] - BSA and Azide free
See all CD3 primary antibodies
DescriptionMouse monoclonal [UCH-T1] to CD3 - BSA and Azide free
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human CD3. (Human infant thymocytes and Sezary cells).
- Molt4 and Jurkat whole cell lysate (ab7899); Human PBMCs.
Clone UCH-T1 is considered a pan T-cell marker - it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemias. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Sézary leukaemias and approximately 70% of acute lymphoblastic leukaemias of T cell origin.
ab215267 is a recombinant monoclonal antibody produced from the original monoclonal with variable regions (i.e. specificity) from the hybridoma.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab215267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 3 - 4 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 19 kDa).|
|Flow Cyt||Use at an assay dependent concentration.|
FunctionThe CD3 complex mediates signal transduction.
Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Sequence similaritiesContains 1 ITAM domain.
- Information by UniProt
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
All lanes : Anti-CD3 antibody [UCH-T1] - BSA and Azide free (ab215267) at 3 µg/ml
Lane 1 : Jurkat cell lysate (in RIPA buffer)
Lane 2 : Molt4 cell lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 19 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Flow cytometric analysis of the dose-dependency (D, F, H) of ab215267 binding to live human PBMCs (B). The gating strategy is shown for control puposes (A, B, C, E, G).
ab215267 has not yet been referenced specifically in any publications.