Anti-CD3 epsilon antibody (ab185811)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD3 epsilon antibody
See all CD3 epsilon primary antibodies -
Description
Rabbit polyclonal to CD3 epsilon -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human CD3 epsilon aa 25-207. The exact sequence is proprietary.
Database link: P07766 -
Positive control
- WB: Jurkat, Raji and MCF-7 cell lysates, Mouse thymus tissue lysate; IHC: human tonsil tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab185811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/2000. | |
| IHC-P | 1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IP | 1/50 - 1/100. |
Target
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 916 Human
- Entrez Gene: 12501 Mouse
- Entrez Gene: 315609 Rat
- Omim: 186830 Human
- SwissProt: P07766 Human
- SwissProt: P22646 Mouse
- Unigene: 3003 Human
- Unigene: 210361 Mouse
see all -
Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (ab185811)Paraffin-embedded human tonsil tissue stained for CD3 epsilon using ab185811 at 1/200 dilution in immunohistochemical analysis.
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Western blot - Anti-CD3 epsilon antibody (ab185811)All lanes : Anti-CD3 epsilon antibody (ab185811) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Raji cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : Mouse thymus tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Exposure time: 5 secondsBlocking buffer: 3% nonfat dry milk in TBST.
Protocols
Datasheets and documents
References
ab185811 has not yet been referenced specifically in any publications.
