Key features and details
- Rabbit polyclonal to CD3 epsilon
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-CD3 epsilon antibody
See all CD3 epsilon primary antibodies
DescriptionRabbit polyclonal to CD3 epsilon
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human CD3 epsilon aa 25-207. The exact sequence is proprietary.
Database link: P07766
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThe CD3 complex mediates signal transduction.
Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain.
- Information by UniProt
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
Paraffin-embedded human tonsil tissue stained for CD3 epsilon using ab185811 at 1/200 dilution in immunohistochemical analysis.
All lanes : Anti-CD3 epsilon antibody (ab185811) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Raji cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : Mouse thymus tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Exposure time: 5 seconds
Blocking buffer: 3% nonfat dry milk in TBST.
ab185811 has not yet been referenced specifically in any publications.