Overview

  • Product name

  • Description

    Rabbit polyclonal to CD3 epsilon
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Hamster, Cow, Cat, Dog, Pig, Monkey, Macaque monkey, Rhesus monkey, Opossum, Hoolock gibbon, Orangutan, Frog, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human CD3 epsilon.
    Database link: P07766

  • Positive control

    • Jurkat cell lysate; mouse thymus lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab187281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-4 µg/mg of lysate.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 23 kDa.
Flow Cyt Use 0.5-1µg for 106 cells.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use a concentration of 1 - 2 µg/ml.

Target

Images

  • All lanes : Anti-CD3 epsilon antibody (ab187281) at 1 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with immunizing peptide
    Lane 3 : Mouse thymus lysate

    Predicted band size: 23 kDa

References

ab187281 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Permeabilization
No
Sample
Tammar wallaby (Macropus eugenii) Cell (PBMC Tasmanian devil (Sarcophilus harrisii) and h)
Specification
PBMC Tasmanian devil (Sarcophilus harrisii) and h
Gating Strategy
MNC gated by FSC/SSC, dead cell excluded by DAPI staining
Preparation
Cell harvesting/tissue preparation method: mononuclear cells separated from heperanised blood using histopaque 1077
Sample buffer: FACS buffer (PBS/0.5% BSA/0.05% azide)

Abcam user community

Verified customer

Submitted Sep 24 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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