Product nameAnti-CD3 epsilon antibody
See all CD3 epsilon primary antibodies
DescriptionRabbit polyclonal to CD3 epsilon
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- Recombinant Human CD3 epsilon protein (ab114153) can be used as a positive control in WB. Tonsil.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab26249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThe CD3 complex mediates signal transduction.
Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain.
- Information by UniProt
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen epsilon polypeptide (TiT3 complex) antibody
All lanes : Anti-CD3 epsilon antibody (ab26249) at 1/500 dilution
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
ab26249 (2µg/ml) staining CD3 epsilon in human spleen.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab26249 has not yet been referenced specifically in any publications.