Key features and details
- Mouse monoclonal [APA1/1] to CD3 epsilon
- Suitable for: Flow Cyt, IP, WB, IHC-Fr, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Product nameAnti-CD3 epsilon antibody [APA1/1]
See all CD3 epsilon primary antibodies
DescriptionMouse monoclonal [APA1/1] to CD3 epsilon
Tested applicationsSuitable for: Flow Cyt, IP, WB, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Purified Human CD3 epsilon isolated from thymus.
- Human T cells stimulated with ab8090
ab134408 recognizes an activation-dependent intracellular epitope of CD3 epsilon. ab134408 can distinguish TCR-stimulated from non-stimulated cells.
Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Purification notesab134408 was purified from cell culture supernatant. Purity >95% by SDS-PAGE.
Primary antibody notesab134408 recognizes an activation-dependent intracellular epitope of CD3 epsilon. ab134408 can distinguish TCR-stimulated from non-stimulated cells.
Matched antibody pairs
Our Abpromise guarantee covers the use of ab134408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min. on ice. Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1% saponine, 5 min. RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 µg/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min. RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. Fixed and permeabilised cells.|
FunctionThe CD3 complex mediates signal transduction.
Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain.
- Information by UniProt
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
ab134408 has not yet been referenced specifically in any publications.