Overview

  • Product name

    Anti-CD3 epsilon antibody [APA1/1]
    See all CD3 epsilon primary antibodies
  • Description

    Mouse monoclonal [APA1/1] to CD3 epsilon
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, IP, WB, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Purified Human CD3 epsilon isolated from thymus.

  • Positive control

    • Human T cells stimulated with ab8090
  • General notes

    ab134408 recognizes an activation-dependent intracellular epitope of CD3 epsilon. ab134408 can distinguish TCR-stimulated from non-stimulated cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab134408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min. on ice. Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1% saponine, 5 min. RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 µg/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min. RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. Fixed and permeabilised cells.

Target

  • Function

    The CD3 complex mediates signal transduction.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

References

ab134408 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab134408.
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