Overview

  • Product name
    Anti-CD3 epsilon antibody [C363.29B] (FITC)
    See all CD3 epsilon primary antibodies
  • Description
    Rat monoclonal [C363.29B] to CD3 epsilon (FITC)
  • Host species
    Rat
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody recognises CD3 epsilon chain.
  • Tested applications
    Suitable for: Flow Cyt, IP, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse CD3 epsilon. IL-4 producing Th2 cell lines including D10.

  • Epitope
    This antibody reacts with an epitope located in the epsilon chain of the CD3/TCR complex.
  • Positive control
    • FC: Cells from BALB/c mouse mesenteric lymph nodes. Mouse splenocytes.

Properties

Applications

Our Abpromise guarantee covers the use of ab24948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 3µg for 106 cells.

In vitro depletion of CD3+ cells and activation of T cells (accessory cells must be provided).
ab114134 - Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

Target

  • Function
    The CD3 complex mediates signal transduction.
  • Sequence similarities
    Contains 1 Ig-like (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD3 epsilon antibody
    • CD3e antibody
    • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3E antigen, epsilon subunit antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule, epsilon (CD3 TCR complex) antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3E_HUMAN antibody
    • IMD18 antibody
    • T cell antigen receptor complex epsilon subunit of T3 antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T3E antibody
    • TCRE antibody
    see all

Images

  • Flow Cytometry analysis of C57BL/6 mouse splenocytes stained with Anti-CD3 epsilon antibody [C363.29B] (FITC) ab24948 (1 µg/106 cells) and Rat Anti-Mouse CD19-PE.

  • Ab24948 (3µg for 106 cells) and anti mouse CD45R/B220 RPE conjugate antibody doubly staining cells from BALB/c mesenteric lymph nodes, gated on small lymphocytes and analyzed using a flow cytometer.

References

This product has been referenced in:
  • Zulkawi N  et al. In vitro characterization and in vivo toxicity, antioxidant and immunomodulatory effect of fermented foods; Xeniji™. BMC Complement Altern Med 17:344 (2017). Flow Cyt ; Mouse . Read more (PubMed: 28666436) »
  • Muraille E  et al. Direct visualization of peptide/MHC complexes at the surface and in the intracellular compartments of cells infected in vivo by Leishmania major. PLoS Pathog 6:e1001154 (2010). Read more (PubMed: 20976202) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your phone call. I can confirm that you can send the products back to us. When you return the goods, please ensure that you follow the procedures as below: - Return in the original Abcam box with the cooler box and ice pack (or similar) within 7 days from today. - Return all documents (or copies of) that arrived with the product (if they’re available – should be invoice, delivery note and datasheet). Please note that we will be unable to issue the credit note if the product is dispatched without the cooler box. However we are not able to accept products returned on dry ice. - Please mark on the documents the reason of return and Ref: - Please return using an over night courier. - You will need to provide the courier with an AirWay Bill – which you can get from the courier, and fill out with our details. - Our address is: Abcam plc, 330 Cambridge Science Park, Milton Road, Cambridge, CB4 0FL When we have received the goods, we will process the return. We will then raise a credit note for the invoice less 20% restocking fee. If you have already paid this invoice, we will reallocate this money to a new invoice for a future order and if there’s any difference in price, we will refund or re-invoice accordingly. For the correct item, please place a new order in the normal methods. Please confirm if you are planning to return the product to us. Important: if the product is not returned to Abcam within next 7 days we will cancel the credit note. PS: We have anti IgG2a and anti IgG2c available in catalogue that are currently in stock. These 2 antibodies exactly match your request. The catalogue numbers are: ab18446 and ab114134

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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