APC Anti-CD3 epsilon antibody [C363.29B (YCD3-1)] (ab25278)
Key features and details
- APC Rat monoclonal [C363.29B (YCD3-1)] to CD3 epsilon
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2b
Overview
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Product name
APC Anti-CD3 epsilon antibody [C363.29B (YCD3-1)]
See all CD3 epsilon primary antibodies -
Description
APC Rat monoclonal [C363.29B (YCD3-1)] to CD3 epsilon -
Host species
Rat -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to CD3 epsilon. IL-4 producing Th2 cell lines including D10
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Epitope
ab25278 recognizes an epitope on the 25kD epsilon chain chain of the CD3/TCR complex. -
General notes
In the presence of Fc receptor-bearing accessory cells, soluble ab25278 can activate primed and naïve T cell in vitro.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Stabilising agent. -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified. -
Primary antibody notes
In the presence of Fc receptor-bearing accessory cells, soluble ab25278 can activate primed and naïve T cell in vitro. -
Clonality
Monoclonal -
Clone number
C363.29B (YCD3-1) -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 1µg for 106 cells.
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Notes |
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Flow Cyt
Use 1µg for 106 cells. |
Target
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12501 Mouse
- SwissProt: P22646 Mouse
- Unigene: 210361 Mouse
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Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab25278 has been referenced in 1 publication.
- Portoles P et al. Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation. J Immunol 142:4169-75 (1989). PubMed: 2470817