Product nameAnti-CD3 epsilon antibody [CA17.2A12]
See all CD3 epsilon primary antibodies
DescriptionMouse monoclonal [CA17.2A12] to CD3 epsilon
Tested applicationsSuitable for: Flow Cyt, IHC-P, IHC-Fr, IP, ICC/IFmore details
Species reactivityReacts with: Dog
Full length native protein (purified) corresponding to Dog CD3 epsilon aa 22-202. Affinity enriched CD3 epsilon isolated from thymocytes and the T cell line CLGL-90.
QDEDFKASDDLTSISPEKRFKVSISGTEVVVTCPDVFGYDNIKWEKNDNL VEGASNRELSQKEFSEVDDSGYYACYADSIKEKSYLYLRARVCANCIEVN LMAVVTIIVADICLTLGLLLMVYYWSKTRKANAKPVMRGTGAGSRPRGQN KEKPPPVPNPDYEPIRKGQQDLYSGLNQRGI
Database link: P27597
- Dog peripheral blood lymphocytes
ab194842 is a valuable flow cytometric and immunohistologic tool for Dog lymphoma detection of T-cell origin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified IgG prepared by affinity chromatography on Protein G.
Our Abpromise guarantee covers the use of ab194842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/25 - 1/50.
10 μl of the suggested working dilution to label 106 cells or 100 μl whole blood.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionThe CD3 complex mediates signal transduction.
Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain.
- Information by UniProt
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
ab194842 has not yet been referenced specifically in any publications.