Anti-CD3 epsilon antibody - Cytoplasmic domain (ab200555)

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Western blot - Anti-CD3 epsilon antibody - Cytoplasmic domain (ab200555)

    Key features and details

    • Goat polyclonal to CD3 epsilon - Cytoplasmic domain
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

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    Anti-CD3 epsilon antibody [EP449E] (ab52959)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-CD3 epsilon antibody - Cytoplasmic domain
      See all CD3 epsilon primary antibodies

    • Description

      Goat polyclonal to CD3 epsilon - Cytoplasmic domain

    • Host species

      Goat

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Cat, Dog, Pig

    • Immunogen

      Synthetic peptide corresponding to Human CD3 epsilon aa 192-205 (C terminal) (Cysteine residue). NP_000724.1.
      Sequence:

      RKGQRDLYSGLNQR


      Database link: P07766
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Recombinant Human CD3 epsilon protein (ab114153) can be used as a positive control in WB. WB: Jurkat and MOLT4 cell lysates.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab200555 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 23 kDa.

    1 hour primary incubation is recommended for this product.

    Target

    Images

    • Western blot - Anti-CD3 epsilon antibody - Cytoplasmic domain (ab200555)
      Western blot - Anti-CD3 epsilon antibody - Cytoplasmic domain (ab200555)
      All lanes : Anti-CD3 epsilon antibody - Cytoplasmic domain (ab200555) at 0.1 µg/ml

      Lane 1 : Jurkat cell lysate
      Lane 2 : MOLT4 cell lysate

      Lysates/proteins at 35 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 23 kDa



      Primary incubation was 1 hour.

    References (0)

    Publishing research using ab200555? Please let us know so that we can cite the reference in this datasheet.

    ab200555 has not yet been referenced specifically in any publications.

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