Anti-CD3 epsilon antibody - Cytoplasmic domain (ab200555)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CD3 epsilon antibody - Cytoplasmic domain
See all CD3 epsilon primary antibodies -
Description
Goat polyclonal to CD3 epsilon - Cytoplasmic domain -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Cat, Dog, Pig
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Immunogen
Synthetic peptide corresponding to Human CD3 epsilon aa 192-205 (C terminal) (Cysteine residue). NP_000724.1.
Sequence:RKGQRDLYSGLNQR
Database link: P07766 -
Positive control
- Recombinant Human CD3 epsilon protein (ab114153) can be used as a positive control in WB. WB: Jurkat and MOLT4 cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab200555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 23 kDa. |
Target
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 281054 Cow
- Entrez Gene: 442981 Dog
- Entrez Gene: 916 Human
- Entrez Gene: 12501 Mouse
- Entrez Gene: 397455 Pig
- Entrez Gene: 100008616 Rabbit
- Entrez Gene: 315609 Rat
- Omim: 186830 Human
see all -
Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
Images
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Western blot - Anti-CD3 epsilon antibody - Cytoplasmic domain (ab200555)All lanes : Anti-CD3 epsilon antibody - Cytoplasmic domain (ab200555) at 0.1 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : MOLT4 cell lysate
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 23 kDaPrimary incubation was 1 hour.
Protocols
Datasheets and documents
References
ab200555 has not yet been referenced specifically in any publications.
