Anti-CD3 epsilon antibody - Cytoplasmic domain (ab200555)
Key features and details
- Goat polyclonal to CD3 epsilon - Cytoplasmic domain
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD3 epsilon antibody - Cytoplasmic domain
See all CD3 epsilon primary antibodies -
Description
Goat polyclonal to CD3 epsilon - Cytoplasmic domain -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Cat, Dog, Pig -
Immunogen
Synthetic peptide corresponding to Human CD3 epsilon aa 192-205 (C terminal) (Cysteine residue). NP_000724.1.
Sequence:RKGQRDLYSGLNQR
Database link: P07766 -
Positive control
- Recombinant Human CD3 epsilon protein (ab114153) can be used as a positive control in WB. WB: Jurkat and MOLT4 cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab200555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 23 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 281054 Cow
- Entrez Gene: 442981 Dog
- Entrez Gene: 916 Human
- Entrez Gene: 12501 Mouse
- Entrez Gene: 397455 Pig
- Entrez Gene: 100008616 Rabbit
- Entrez Gene: 315609 Rat
- Omim: 186830 Human
see all -
Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
Images
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All lanes : Anti-CD3 epsilon antibody - Cytoplasmic domain (ab200555) at 0.1 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : MOLT4 cell lysate
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 23 kDaPrimary incubation was 1 hour.
Datasheets and documents
References (0)
ab200555 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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